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Literature summary for 2.7.7.52 extracted from

  • Zhu, L.; Hu, Q.; Cheng, L.; Jiang, Y.; Lv, M.; Liu, Y.; Li, F.; Shi, Y.; Gong, Q.
    Crystal structure of Arabidopsis terminal uridylyl transferase URT1 (2020), Biochem. Biophys. Res. Commun., 524, 490-496 .
    View publication on PubMed
Search for more literature for 2.7.7.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Gold (DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme D547A, sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.5, 10% (w/v) PEG 6000 and 5% (w/v) 2-methyl-2,4-pentanediol Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D547A catalytically dead mutant Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + mRNAn Arabidopsis thaliana for mRNA, URT1 is the main enzyme to uridylate the majority of mRNA and repairs their deadenylated ends to restore the binding site for poly(A) binding protein. Enzyme HESO1, on the other hand, targets mostly the mRNAs with very short oligo(A) tails and fails in fulfilling the same task diphosphate + mRNAn+1
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 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O64642 URT1
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + miR158an miR158a has the sequence 5'-Cy5-UCCCAAAUGUAGACAAAGCA-3' Arabidopsis thaliana diphosphate + miR158an+1
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 ?
UTP + miRNAn the enzyme URT1 plays a redundant but important role in miRNA uridylation when HESO1 is absent Arabidopsis thaliana diphosphate + miRNAn+1
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 ?
UTP + mRNAn for mRNA, URT1 is the main enzyme to uridylate the majority of mRNA and repairs their deadenylated ends to restore the binding site for poly(A) binding protein. Enzyme HESO1, on the other hand, targets mostly the mRNAs with very short oligo(A) tails and fails in fulfilling the same task Arabidopsis thaliana diphosphate + mRNAn+1
-
 ?
UTP + mRNAn for mRNA, URT1 is the main enzyme to uridylate the majority of mRNA (70-80%) and repairs their deadenylated ends to restore the binding site for poly(A) binding protein. Enzyme HESO1, on the other hand, targets mostly the mRNAs with very short oligo(A) tails and fails in fulfilling the same task Arabidopsis thaliana diphosphate + mRNAn+1
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 ?

Synonyms

Synonyms Comment Organism
HESO1
-
 Arabidopsis thaliana
terminal uridylyl transferase
-
 Arabidopsis thaliana
TUTase
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 Arabidopsis thaliana
URT1
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 Arabidopsis thaliana
UTP: RNA uridylyltransferase
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 Arabidopsis thaliana