EC Number   |
Protein Variants |
Reference |
|---|
   2.7.1.127 | A187S |
crystal structure determination and analysis |
662827 |
| 2.7.1.127 | D262A |
14% of wild-type enzyme activity |
662827 |
| 2.7.1.127 | D262N |
12% of wild-type enzyme activity |
662827 |
| 2.7.1.127 | D416A |
inactive mutant |
662827 |
| 2.7.1.127 | D423N |
crystal structure determination and analysis |
662828 |
| 2.7.1.127 | K199A |
80% of wild-type enzyme activity |
662827 |
| 2.7.1.127 | K262A |
the green fluorescent protein-inositol 1,4,5-trisphosphate 3-kinase A point mutant is generated by site-directed mutagenesis, point mutant lacks kinase activity |
699694 |
| 2.7.1.127 | K268E |
site-directed mutagenesis, activity is similar to the wild-type enzyme, the sensitivity to inhibitor 3',4',7,8-tetrahydroxyflavone is highly reduced compared to the wild-type enzyme |
662346 |
| 2.7.1.127 | K272D |
site-directed mutagenesis, activity is similar to the wild-type enzyme, the sensitivity to inhibitor 3',4',7,8-tetrahydroxyflavone is highly reduced compared to the wild-type enzyme |
662346 |
| 2.7.1.127 | K336Q |
By substituting lysine 336, involving in ATP binding and located in the C-lobe, an inhibitor-resistant IP3K-A with full enzymatic activity and normal substrate affinity is created. |
687650 |
