EC Number   |
Protein Variants |
Reference |
|---|
    2.6.1.42 | A234D |
site-directed mutagenesis of strain BY4741, both deletion mutant bat1DELTA and point mutant bat1A234D cells exhibit the same phenotypes relative to the wild-type Bat1 strain, namely, a repressive growth rate in the logarithmic phase, decreases in intracellular valine and leucine content in the logarithmic and stationary growth phases, respectively, and an increase in fusel alcohol content in culture medium, and a decrease in the carbon dioxide productivity. These results indicate that amino acid change from Ala to Asp at position 234 leads to a functional impairment of Bat1, although homology modeling suggests that Asp234 in the variant Bat1 does not inhibit enzymatic activity directly |
-, 758690 |
| 2.6.1.42 | C221S |
the mutant shows strongly decreased kcat values compared to the wild type enzyme |
702237 |
| 2.6.1.42 | C235S |
the mutant enzyme exhibits kcat values that are within 20-30% of wild type BCATc values, but also have lower Km values, hence higher calculated kcat/Km values than those of the wild type enzyme |
702237 |
| 2.6.1.42 | C242S |
the mutant shows strongly decreased kcat values compared to the wild type enzyme |
702237 |
| 2.6.1.42 | C293S |
the C293S mutant and wild type BCATc have similar kinetic constants with L-valine, L-leucine, and L-glutamate |
702237 |
| 2.6.1.42 | C315A |
analysis of the PLP-bound mutant enzyme crystal structure, ODB ID 2HG8 |
758657 |
| 2.6.1.42 | C315A |
contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity |
674728 |
| 2.6.1.42 | C315A |
site-directed mutagenesis, mutation of the redox sensor (Cys315) results in a significant loss of activity compared to wild-type |
758607 |
| 2.6.1.42 | C315A/C318A |
contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity |
674728 |
| 2.6.1.42 | C318A |
contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity |
674728 |
