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Literature summary for 2.6.1.42 extracted from

  • Yennawar, N.H.; Islam, M.M.; Conway, M.; Wallin, R.; Hutson, S.M.
    Human mitochondrial branched chain aminotransferase isozyme: structural role of the CXXC center in catalysis (2006), J. Biol. Chem., 281, 39660-39671.
    View publication on PubMed
Search for more literature for 2.6.1.42

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 22-30% polyethlylene glycol 1500, 100 mM HEPES (pH 6.9-7.2), and 20 mM dithiothreitol Homo sapiens

Protein Variants

Protein Variants Comment Organism
C315A contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity Homo sapiens
C315A/C318A contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity Homo sapiens
C318A contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens O15382
-
-

Oxidation Stability

Oxidation Stability Organism
the activity of oxidized BCAT with 0.5 mM hydrogen peroxide for 2 h at 25°C shows 99.5% inhibition Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA acid resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + 2-oxoglutarate
-
 Homo sapiens 2-oxoglutarate + L-glutamate
-
 r
L-isoleucine + 2-oxoglutarate
-
 Homo sapiens 3-methyl-2-oxopentanoate + L-glutamate
-
 r
L-leucine + 2-oxoglutarate
-
 Homo sapiens 4-methyl-2-oxopentanoate + L-glutamate
-
 r
L-valine + 2-oxoglutarate
-
 Homo sapiens 3-methyl-2-oxobutanoate + L-glutamate
-
 r

Synonyms

Synonyms Comment Organism
BcaT
-
 Homo sapiens
branched chain aminotransferase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50.1
-
 L-glutamate mutant enzyme C315A/C318A Homo sapiens
52
-
 L-glutamate mutant enzyme C315A Homo sapiens
60.8
-
 L-leucine mutant enzyme C315A/C318A Homo sapiens
65
-
 L-leucine mutant enzyme C315A Homo sapiens
70.5
-
 L-isoleucine mutant enzyme C315A/C318A Homo sapiens
72
-
 L-isoleucine mutant enzyme C315A Homo sapiens
188
-
 L-valine mutant enzyme C315A Homo sapiens
197
-
 L-valine mutant enzyme C315A/C318A Homo sapiens
230
-
 L-valine mutant enzyme C318A Homo sapiens
250
-
 2-oxoglutarate mutant enzyme C315A Homo sapiens
265
-
 L-glutamate mutant enzyme C318A Homo sapiens
270
-
 2-oxoglutarate mutant enzyme C315A/C318A Homo sapiens
277
-
 L-valine wild type enzyme Homo sapiens
290
-
 L-glutamate wild type enzyme Homo sapiens
310
-
 L-leucine mutant enzyme C318A Homo sapiens
319
-
 L-isoleucine mutant enzyme C318A Homo sapiens
337
-
 L-leucine wild type enzyme Homo sapiens
340
-
 2-oxoglutarate wild type enzyme Homo sapiens
360
-
 2-oxoglutarate mutant enzyme C318A Homo sapiens
371
-
 L-isoleucine wild type enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens