EC Number   |
Protein Variants |
Reference |
|---|
    2.5.1.96 | more |
construction of C40 carotenoid synthase pathway in Escherichia coli by expression of crtE encoding GGPP synthase, crtB encoding phytoene synthase, and crtI encoding phytoene desaturase, all from Erwinia uredovora. Replacement of phytoene synthase crtB by dehydrosqualene synthase crtM results in negligible ability to synthesize the C40 product. Some crtM mutants perform comparably to crtB in an in vivo C40 pathway. These mutants show significant variation in performance in their original C30 pathway, indicating the emergence of enzymes with broadened substrate specificity as well as those with shifted specificity |
-, 715351 |
| 2.5.1.96 | Y248A |
crystallization data of complex with zaragozic acid A |
722759 |
| 2.5.1.96 | Q33R/D34N/S38N |
directed evolution of human Q33R/D34N/S38N mutant squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by replacement of hsqs in pAC-hsqs with the Saccharomyces mutant gene ysqs G856A/G1125A/T1128C/T1176C |
738235 |
| 2.5.1.96 | more |
directed evolution of squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by mutation gene tsqs replacement with the human gene mutant Q33R/D34N/S38N/G856A/G1125A/T1128C/T1176C |
738235 |
| 2.5.1.96 | G856A/G1125A/T1128C/T1176C |
directed evolution of squalene synthase, EC 2.5.1.21, in an attempt to mimic the activity of dehydroqsqualene synthase, EC 2.5.1.96, by mutation of gene ysqs |
738235 |
| 2.5.1.96 | more |
expression of CrtM in Escherichia coli leads to synthesis of diapophytoene from pre-existing farnesyl diphosphate |
-, 713989 |
| 2.5.1.96 | A726G, A850G |
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity |
715351 |
| 2.5.1.96 | F26L/E149G |
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity |
-, 715351 |
| 2.5.1.96 | F26L/F267S |
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity |
715351 |
| 2.5.1.96 | F26L/V43M |
mutant with phytoene synthase activity, decrease in dehydrosqualene synthase activity |
-, 715351 |
