EC Number   |
Protein Variants |
Reference |
|---|
  2.5.1.154 | Cys79A |
mutation in the HX11CCX2C(83) motif required for the tight binding of the divalent metal, substitution causes a large decrease in the affinity of EutT for Co(II) |
762727 |
| 2.5.1.154 | Cys80A |
mutation in the HX11CCX2C(83) motif required for the tight binding of the divalent metal, inactive |
762727 |
| 2.5.1.154 | Cys83A |
mutation in the HX11CCX2C(83) motif required for the tight binding of the divalent metal, large negative effect on enzyme activity, only capable of very slow turnover |
762727 |
| 2.5.1.154 | D110N |
formation of a salt bridge with residue R200 |
-, 758827 |
| 2.5.1.154 | D110R |
formation of a salt bridge with residue R200 |
758827 |
| 2.5.1.154 | D110R/R200D |
residue form a salt bridge |
758827 |
| 2.5.1.154 | E207Q |
increase of about 50fold in the Khalf for ATP |
758827 |
| 2.5.1.154 | F72H |
replacement affects access to the substrate-binding site |
758827 |
| 2.5.1.154 | F72Y |
replacement affects access to the substrate-binding site |
758827 |
| 2.5.1.154 | His67A |
mutation in the HX11CCX2C(83) motif required for the tight binding of the divalent metal, at most 1% of the divalent metal sites are occupied |
762727 |
