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Literature summary for 2.5.1.154 extracted from
- Mutational and functional analyses of substrate binding and catalysis of the Listeria monocytogenes EutT ATP Co(I)rrinoid adenosyltransferase (2020), Biochemistry, 59, 1124-1136 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D110N | formation of a salt bridge with residue R200 | Listeria monocytogenes serotype 1/2a |
| D110R | formation of a salt bridge with residue R200 | Listeria monocytogenes serotype 1/2a |
| D110R/R200D | residue form a salt bridge | Listeria monocytogenes serotype 1/2a |
| E207Q | increase of about 50fold in the Khalf for ATP | Listeria monocytogenes serotype 1/2a |
| F72H | replacement affects access to the substrate-binding site | Listeria monocytogenes serotype 1/2a |
| F72Y | replacement affects access to the substrate-binding site | Listeria monocytogenes serotype 1/2a |
| K98Q | increase of about 22fold in the Khalf for ATP | Listeria monocytogenes serotype 1/2a |
| additional information | mutation in EutT to corresponding residues in Lactobacillus reuteri PduO, Ec 2.5.1.17, associated with ATP binding and formation of an intersubunit salt bridge. The equivalent residues in EutT enzyme affect ATP binding | Listeria monocytogenes serotype 1/2a |
| N231L | no detectable activity in vitro | Listeria monocytogenes serotype 1/2a |
| R200D | formation of a salt bridge with residue D110 | Listeria monocytogenes serotype 1/2a |
| R200K | formation of a salt bridge with residue D110 | Listeria monocytogenes serotype 1/2a |
| R204K | no detectable activity in vitro | Listeria monocytogenes serotype 1/2a |
| T88V | increase of about 6fold in the Khalf for ATP | Listeria monocytogenes serotype 1/2a |
| W238A | replacement affects binding of the cobalamin substrate to the active site, mutant strain fails to synthesize enough adenosylcobalamin to support growth | Listeria monocytogenes serotype 1/2a |
| W238H | replacement affects binding of the cobalamin substrate to the active site, mutant strain fails to synthesize enough adenosylcobalamin to support growth | Listeria monocytogenes serotype 1/2a |
| W238L | replacement affects binding of the cobalamin substrate to the active site, mutant strain fails to synthesize enough adenosylcobalamin to support growth | Listeria monocytogenes serotype 1/2a |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0042 | - |
cob(II)alamin | wild-type, Khalf value, Hill coefficient 1.9, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a |  |
| 0.0044 | - |
ATP | wild-type, Khalf value, Hill coefficient 2.3, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0045 | - |
ATP | mutant R200K, Khalf value, Hill coefficient 1.3, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0053 | - |
ATP | mutant D110N, Khalf value, Hill coefficient 1.2, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0092 | - |
cob(II)alamin | mutant D110N, Khalf value, Hill coefficient 2.5, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0094 | - |
cob(II)alamin | mutant R200K, Khalf value, Hill coefficient 1.9, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0105 | - |
cob(II)alamin | mutant T88V, Khalf value, Hill coefficient 1.8, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0275 | - |
ATP | mutant T88V, Khalf value, Hill coefficient 1.2, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0427 | - |
cob(II)alamin | mutant K98Q, Khalf value, Hill coefficient 1.7, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0448 | - |
cob(II)alamin | mutant E207Q, Khalf value, Hill coefficient 2.4, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.0954 | - |
ATP | mutant K98Q, Khalf value, Hill coefficient 0.9, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.229 | - |
ATP | mutant E207Q, Khalf value, Hill coefficient 1.7, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Listeria monocytogenes serotype 1/2a | A0A0H3GFM5 | - |
- |
| Listeria monocytogenes serotype 1/2a 10403S | A0A0H3GFM5 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ATP + 2 cob(II)alamin + a reduced flavoprotein | - |
Listeria monocytogenes serotype 1/2a | 2 diphosphate + 2 phosphate + 2 adenosylcob(III)alamin + an oxidized flavoprotein | - |
? | |
| 2 ATP + 2 cob(II)alamin + a reduced flavoprotein | - |
Listeria monocytogenes serotype 1/2a 10403S | 2 diphosphate + 2 phosphate + 2 adenosylcob(III)alamin + an oxidized flavoprotein | - |
? | |
| 2 ATP + 2 [corrinoid adenosyltransferase]-cob(I)alamin | - |
Listeria monocytogenes serotype 1/2a | 2 diphosphate + 2 phosphate + 2 adenosylcob(III)alamin + 2 [corrinoid adenosyltransferase] | - |
? | |
| 2 ATP + 2 [corrinoid adenosyltransferase]-cob(I)alamin | - |
Listeria monocytogenes serotype 1/2a 10403S | 2 diphosphate + 2 phosphate + 2 adenosylcob(III)alamin + 2 [corrinoid adenosyltransferase] | - |
? | |
| 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] | - |
Listeria monocytogenes serotype 1/2a | 2 [corrinoid adenosyltransferase]-cob(II)alamin | - |
? | |
| 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] | - |
Listeria monocytogenes serotype 1/2a 10403S | 2 [corrinoid adenosyltransferase]-cob(II)alamin | - |
? | |
| a reduced flavoprotein + 2 [corrinoid adenosyltransferase]-cob(II)alamin | - |
Listeria monocytogenes serotype 1/2a | an oxidized flavoprotein + 2 [corrinoid adenosyltransferase]-cob(I)alamin | - |
? | |
| a reduced flavoprotein + 2 [corrinoid adenosyltransferase]-cob(II)alamin | - |
Listeria monocytogenes serotype 1/2a 10403S | an oxidized flavoprotein + 2 [corrinoid adenosyltransferase]-cob(I)alamin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EutT | - |
Listeria monocytogenes serotype 1/2a |
| LMRG_00627 | - |
Listeria monocytogenes serotype 1/2a |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
cob(II)alamin | mutant D110N, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.06 | - |
cob(II)alamin | mutant R200K, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.07 | - |
ATP | mutant D110N, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.1 | - |
ATP | mutant R200K, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.17 | - |
ATP | mutant K98Q, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.18 | - |
ATP | mutant E207Q, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.18 | - |
cob(II)alamin | mutant K98Q, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.21 | - |
cob(II)alamin | wild-type, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.22 | - |
cob(II)alamin | mutant E207Q, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.24 | - |
ATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.26 | - |
cob(II)alamin | mutant T88V, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 0.27 | - |
ATP | mutant T88V, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
ATP | mutant E207Q, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 1.8 | - |
ATP | mutant K98Q, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 4.2 | - |
cob(II)alamin | mutant K98Q, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 4.9 | - |
cob(II)alamin | mutant E207Q, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 5.8 | - |
cob(II)alamin | mutant D110N, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 6.5 | - |
cob(II)alamin | mutant R200K, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 9.8 | - |
ATP | mutant T88V, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 14 | - |
ATP | mutant D110N, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 22 | - |
ATP | mutant R200K, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 25 | - |
cob(II)alamin | mutant T88V, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 50 | - |
cob(II)alamin | wild-type, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
| 54 | - |
ATP | wild-type, pH not specified in the publication, temperature not specified in the publication | Listeria monocytogenes serotype 1/2a | |
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