EC Number |
Protein Variants |
Reference |
---|
2.4.1.336 | D147Q |
mutation significantly reduces the activity and blocks temperature-dependent activation |
703718 |
2.4.1.336 | D200A |
mutation significantly reduces the activity and blocks temperature-dependent activation |
703718 |
2.4.1.336 | K67A/R70A |
mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles |
736087 |
2.4.1.336 | K67A/R70A/R83A/K84A |
mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles |
736087 |
2.4.1.336 | K76A/R77A |
mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles |
736087 |
2.4.1.336 | L62A/V63A/V66A |
mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles |
736087 |
2.4.1.336 | more |
deletion of 9, 19 or 29 amino acids of the C-terminal tail destroys the activity in vitro. The ability to generate internal vesicles is dirupted in mutants lacking 19 and 29 amino acids |
736087 |
2.4.1.336 | R329A |
mutation significantly reduces the activity and blocks temperature-dependent activation |
703718 |
2.4.1.336 | R331A |
mutation significantly reduces the activity and blocks temperature-dependent activation |
703718 |
2.4.1.336 | R63A/K64A |
mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles |
736087 |