Information on EC 2.4.1.336 - monoglucosyldiacylglycerol synthase

beta-monoglucosyldiacylglycerol synthase

MGD1

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MgdA

MGS

monoglucosyldiacylglycerol synthase

Sll1377

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UDP-glucose:1,2-diacylglycerol 3-beta-D-glucosyltransferase

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galactolipid biosynthesis II, type I lipoteichoic acid biosynthesis (S. aureus)

UDP-alpha-D-glucose + a 1,2-diacyl-sn-glycerol = UDP + a 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol

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KEGG

Glycerolipid metabolism

MetaCyc

UDP-alpha-D-glucose:1,2-diacyl-sn-glycerol 3-beta-D-glucosyltransferase

The enzymes from cyanobacteria are involved in the biosynthesis of galactolipids found in their photosynthetic membranes. The enzyme belongs to the GT2 family of configuration-inverting glycosyltranferases [2]. cf. EC 2.4.1.337, 1,2-diacylglycerol 3-alpha-glucosyltransferase.

UDP-alpha-D-glucose + 1,2-dipalmitoyl-sn-glycerol

UDP + 1,2-dipalmitoyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol

UDP-alpha-D-glucose + 1-oleoyl-2-palmitoyl-sn-glycerol

UDP + 1-oleoyl-2-palmitoyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol

UDP-glucose + 1,2-diacyl-sn-glycerol

UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol

UDPglucose + 1,2-diacylglycerol

UDP + 3-D-glucosyl-1,2-diacylglycerol

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-

?

UDPglucose + 1,2-dipalmitoylglycerol

UDP + 3-D-glucosyl-1,2-dipalmitoylglycerol

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-

?

UDPglucose + 1-oleoyl-2-palmitoylglycerol

UDP + 3-D-glucosyl-1-oleoyl-2-palmitoylglycerol

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-

?

UDPglucose + 1-stearoyl-2-palmitoylglycerol

UDP + 3-D-glucosyl-1-stearoyl-2-palmitoylglycerol

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-

?

additional information

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5 entries

UDP-alpha-D-glucose + 1,2-dipalmitoyl-sn-glycerol

UDP + 1,2-dipalmitoyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol

UDP-alpha-D-glucose + 1-oleoyl-2-palmitoyl-sn-glycerol

UDP + 1-oleoyl-2-palmitoyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol

UDP-glucose + 1,2-diacyl-sn-glycerol

UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol

additional information

?

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Mg2+

digalactosyldiacylglycerol

inhibition by the final products of galactolipid synthesis

EDTA

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monogalactosyldiacylglycerol

inhibition by the final products of galactolipid synthesis

sulfoquinovosyldiacylglycerol

additional information

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0.045

UDPglucose

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7

-

8

assay at

5 - 8.7

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pH 5.0: about 45% of maximal activity, pH 8.7: about 50% of maximal activity

30

assay at

45

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28 - 55

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28Â°C: 50% of maximal activity, 55Â°C: 35% of maximal activity

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UniProt

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Anabaena sp. PCC 7120

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-

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UniProt

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-

-

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membrane

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metabolism

the enzyme is involved in the lipid synthesis in Synechocystis, pathway overview

physiological function

5 entries

structural model displays the characteristic double Rossmann fold of the GT-B superfamily, with the active site located in the cleft between the two domains. The C-terminal tail stretches back to and interacts with the N domain, perhaps acting as a tether that maintains close contacts between the two domains

R83A/K84A

R80A/K81A

mutation in the binding alpha helix, mutant is active and able to generate internal vesicles.

R63A/K64A

mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles

L62A/V63A/V66A

mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles

K76A/R77A

mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles

K67A/R70A/R83A/K84A

mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles

K67A/R70A

mutation in the binding alpha helix, activity is dramatically reduced or even abolished, mutant is able to generate internal vesicles

D147Q