EC Number |
Protein Variants |
Reference |
---|
2.3.2.25 | C91A |
site-directed mutagenesis of an active site residue, the mutation eliminates the ability of Ube2W to transfer ubiquitin (Ub) to substrates while still allowing Ube2W to bind substrates, it disrupts Ube2W-mediated ubiquitination |
-, 759848 |
2.3.2.25 | more |
absence of Ube2W increases soluble, monomeric mutant huntingtin (HTT) in a knock-in mouse model of Huntington's disease. The absence of Ube2W in HdhQ200 KI mice significantly increases levels of soluble monomeric mHTT while reducing insoluble oligomeric species |
-, 759848 |
2.3.2.25 | more |
generation of E2-knockdown macrophages, siRNA knockdown of Ube2o expression in engineered RAW-RA cells, Ube2o knockdown efficiency is measured by quantitative PCR |
759122 |
2.3.2.25 | more |
specific downregulation of enzyme UBE2W in spermatogenic cells by murine UBE2W-specific shRNA. UBE2W downregulation promotes cell apoptosis and correlates with hypospermatogenesis. UBE2W upregulation is performed by a recombinant lentivirus containing murine UBE2W |
-, 758638 |
2.3.2.25 | more |
to obtain homogenous and pure ubiquitin (Ub)-modified alphasynuclein (alphaS) and tauK18, engineered constructs that expressed fusion proteins with a single Ub moiety immediately before the first residue of alphaS or tauK18 (Ub-alphaS and Ub-tauK18) are genetically engineered. These engineered N-terminal Ub-fusion proteins are protected from deubiquitination by a Gly76Ser substitution of the C-terminal residue of Ub. No filamentous aggregates from Ub-alphaS are detected under TEM, thus, the morphology of filamentous aggregates is affected by N-terminal Ub modification. Meanwhile, oligomers from both tauK18 and Ub-tauK18 are reproducibly detected early in the aggregation process. An apparent reduction of the fraction of soluble oligomers with time is detected in both unmodified and Ub-modified tauK18 beyond 50 and 70 h, respectively. Comparisons of modified and unmodified proteins' aggregation behaviour, overview. Proteasomes are able to target Ub-modified aggregates |
759630 |
2.3.2.25 | W144E |
site-directed mutagenesis, the mutation eliminates substrate binding and disrupts Ube2W-mediated ubiquitination |
-, 759848 |