EC Number |
Protein Variants |
Reference |
---|
2.3.1.258 | more |
generation of mutants MtRimI4-158, MtRimI1-153, MtRimI4-153, MtRimIC21A, and of the final construct MtRimIC21A4-153, MtRimIC21A4-153 has almost identical enzymatic activity compared to MtRimI, indicating insignificant influence of the recombinant variations on enzymatic functions. The 2D 1H-15N heteronuclear single quantum coherence spectrum of tRimIC21A4-153 exhibits wider chemical shift dispersion and favorable peak isolation, indicating that MtRimIC21A4-153 is amendable for further structural determination. Moreover, bio-layer interferometry experiments show that MtRimIC21A4-153 possesses similar micromolar affinity to full-length MtRimI for binding the hexapeptide substrate Ala-Arg-Tyr-Phe-Arg-Arg. Structure comparison of wild-type MtRimI and mutant MtRimIC21A4-153 |
-, 755712 |
2.3.1.258 | H112A |
inactive |
720002 |
2.3.1.258 | more |
N-terminal analyses comparing wild-type and scNaa50 deletion strains of Saccharomyces cerevisiae |
-, 758492 |
2.3.1.258 | H112A |
NMR spectroscopy using the catalytically inactive hNaa50p mutant |
720002 |
2.3.1.258 | more |
recombinant GST-tagged hNaa50 fails to pull down Schizosaccharomyces pombe SpNatA and hNaa50 and SpNatA cannot form a stoichiometric complex |
-, 758493 |
2.3.1.258 | F27A |
site-directed mutagenesis, inactive mutant |
719976 |
2.3.1.258 | F35A |
site-directed mutagenesis, inactive mutant |
719976 |
2.3.1.258 | H112A |
site-directed mutagenesis, inactive mutant |
719976 |
2.3.1.258 | H112F |
site-directed mutagenesis, inactive mutant |
719976 |
2.3.1.258 | Y139A |
site-directed mutagenesis, inactive mutant |
719976 |