Application | Comment | Organism |
---|---|---|
medicine | Naa50p is a therapeutic anti-cancer target, the structure of the ternary Naa50p complex also provides a molecular scaffold for the design of NAT-specific small molecule inhibitors with possible therapeutic applications | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
expression of GST-tagged full-length Naa50p with a TEV protease-cleavable site in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
enzyme bound to a native substrate peptide fragment (MLGPEGGRWG) and CoA, hanging drop vapor diffusion method, using 16% (w/v) PEG 8000, 20% (v/v) glycerol, and 40 mM potassium phosphate (monobasic, pH 5.0) | Homo sapiens |
Naa50p bound to a native substrate peptide fragment and CoA, hanging drop vapor diffusion method, mixing of 12 mg/ml protein in 25 mM HEPES, pH 7.5, 100 mM NaCl, and 10mM dithiothreitol, with peptide MLGPEGGRWG solution, and crystallization solution, containing 16% PEG 8000, 20% glycerol, and 40 mM potassium phosphate, pH 5.0, in a 1:3:3 molar ratio, 20°C, 1-3 days, X-ray diffraction structure determination and analysis at 2.75 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F27A | site-directed mutagenesis, inactive mutant | Homo sapiens |
F27A | the mutant shows less than 10% of wild type catalytic efficiency | Homo sapiens |
F35A | site-directed mutagenesis, inactive mutant | Homo sapiens |
F35A | the mutant shows less than 10% of wild type catalytic efficiency | Homo sapiens |
H112A | site-directed mutagenesis, inactive mutant | Homo sapiens |
H112A | the mutant shows less than 10% of wild type catalytic efficiency | Homo sapiens |
H112F | site-directed mutagenesis, inactive mutant | Homo sapiens |
H112F | the mutant shows less than 10% of wild type catalytic efficiency | Homo sapiens |
I142A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
I142A | the mutant shows 42.2% of wild type catalytic efficiency | Homo sapiens |
P28A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
P28A | the mutant shows less 13.4% of wild type catalytic efficiency | Homo sapiens |
V29A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
V29A | the mutant shows 12.3% of wild type catalytic efficiency | Homo sapiens |
Y139A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y139A | the mutant shows less than 10% of wild type catalytic efficiency | Homo sapiens |
Y31A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y31A | the mutant shows less than 10% of wild type catalytic efficiency | Homo sapiens |
Y73A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y73A | the mutant shows less than 10% of wild type catalytic efficiency | Homo sapiens |
Y73F | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y73F | the mutant shows less than 10% of wild type catalytic efficiency | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + MLGPEGGRWG | Homo sapiens | - |
CoA + Ac-MLGPEGGRWG | - |
? | |
acetyl-CoA + peptide | Homo sapiens | - |
CoA + Nalpha-acetylpeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q9GZZ1 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap SP column chromatography, and Superdex 75 gel filtration | Homo sapiens |
recombinant GST-tagged full-length Naa50p from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and cleavage of the GST-tag by TEV protease, followed by ion exchange chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + MLGPEGGRWG | - |
Homo sapiens | CoA + Ac-MLGPEGGRWG | - |
? | |
acetyl-CoA + MLGPEGGRWGRPVGRRRRP | - |
Homo sapiens | acetyl-CoA + Nalpha-acetyl-MLGPEGGRWGRPVGRRRRP | - |
? | |
acetyl-CoA + peptide | - |
Homo sapiens | CoA + Nalpha-acetylpeptide | - |
? | |
acetyl-CoA + peptide | peptide substrate binding structure, overview | Homo sapiens | CoA + Nalpha-acetylpeptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Naa50p | - |
Homo sapiens |
Naa50p | subunit of NatA | Homo sapiens |
Naa50p (NAT5/SAN) N-terminal acetyltransferase complex | - |
Homo sapiens |
NAT | - |
Homo sapiens |
NAT5/SAN | - |
Homo sapiens |
NatA | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Homo sapiens |