EC Number |
Protein Variants |
Reference |
---|
2.3.1.137 | C323M |
site-directed mutagenesis, crystal structure comparison with the wild-type enzyme, altered acyl-CoA substrate specificity compared to the wild-type enzyme, highly increased specificity for octanoyl-CoA |
659511 |
2.3.1.137 | G553M |
site-directed mutagenesis, highly reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme |
659511 |
2.3.1.137 | G553M |
site-directed mutagenesis, the mutant enzyme shows altered substrate specificity: highly reduced activity with medium- and long-chain acyl-CoAs and increased activity with acetyl-CoA and butanoyl-CoA compared to the wild-type enzyme |
659372 |
2.3.1.137 | M335A |
site-directed mutagenesis, reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme |
659511 |
2.3.1.137 | M335V |
site-directed mutagenesis, crystal structure comparison with the wild-type enzyme, highly reduced activity with altered acyl-CoA substrate specificity compared to the wild-type enzyme |
659511 |
2.3.1.137 | more |
the carnitine acetyl-transferase, specific for acetyl-CoA/short-chain acyl-CoAs, can be modified to an enzyme with elevated carnitine octanoyltransferase activity by mutation of Met564 to Gly, overview |
659372 |
2.3.1.137 | R518N |
site-directed mutagenesis, mutant shows 1650fold increased Km for L-carnitine, only slightly affected Km for acyl-CoA and kcat compared to the wild-type enzyme |
657546 |
2.3.1.137 | S552A |
site-directed mutagenesis, mutant shows 17fold increased Km for L-carnitine compared to the wild-type enzyme |
657546 |
2.3.1.137 | S554X |
site-directed mutagenesis, mutant shows 10fold decreased kcat compared to the wild-type enzyme |
657546 |