Leibniz Institute DSMZ
DSMZ Digital Diversity
Login
Classic view
All enzymes
Enzyme history
BRENDA support
Any feedback?
Please rate this page
(search_result.php)
😁
😐
😡
(
0
/150)
Send feedback
BRENDA support
Refine search
Search Protein Variants
Protein Variants:
show
10
50
100
results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search
Recommended Name:
EC Number:
contains
exact
begins with
ends with
use * as joker
Commentary:
contains
exact
begins with
ends with
use * as joker
Organism
:
contains
exact
begins with
ends with
use * as joker
Reference:
contains
exact
begins with
ends with
use * as joker
Search term:
Results
1
-
2
of
2
download as CSV
download all results as CSV
EC Number
Protein Variants
Commentary
Reference
2.1.1.337
E204G
mutation to the corresponding residue of Coptis japonica coclaurine N-methyltransferase. The mutation has similarly modest (negative) effects on Km and kcat for (S)-N-methylcoclaurine, but with respect to (S)-coclaurine has a substantial (positive) effect only on kcat
757949
2.1.1.337
G204E
the mutation results in an enzyme with reduced canonical reticuline N-methyltransferase activity and increased canonical coclaurine N-methyltransferase activity. The mutant enzyme shows activity with (S)-coclaurine, which is modestly better than with (S)-N-methylcoclaurine but worse than with (S)-reticuline. A G204E substitution has no appreciable effect on Km for (S)-coclaurine but increases Km for (S)-N-methylcoclaurine approximately 4fold. kcat for (S)-coclaurine is 10fold greater while that for (S)-N-methylcoclaurine decreases 4fold. The G204E substitution has similarly modest (negative) effects on Km and kcat for (S)-N-methylcoclaurine, but with respect to (S)-coclaurine has a substantial (positive) effect only on kcat
757949
Results
1
-
2
of
2
download as CSV
download all results as CSV