Protein Variants | Comment | Organism |
---|---|---|
E204G | mutation to the corresponding residue of Coptis japonica coclaurine N-methyltransferase. The mutation has similarly modest (negative) effects on Km and kcat for (S)-N-methylcoclaurine, but with respect to (S)-coclaurine has a substantial (positive) effect only on kcat | Papaver somniferum |
G204E | the mutation results in an enzyme with reduced canonical reticuline N-methyltransferase activity and increased canonical coclaurine N-methyltransferase activity. The mutant enzyme shows activity with (S)-coclaurine, which is modestly better than with (S)-N-methylcoclaurine but worse than with (S)-reticuline. A G204E substitution has no appreciable effect on Km for (S)-coclaurine but increases Km for (S)-N-methylcoclaurine approximately 4fold. kcat for (S)-coclaurine is 10fold greater while that for (S)-N-methylcoclaurine decreases 4fold. The G204E substitution has similarly modest (negative) effects on Km and kcat for (S)-N-methylcoclaurine, but with respect to (S)-coclaurine has a substantial (positive) effect only on kcat | Papaver somniferum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0508 | - |
(S)-Coclaurine | pH 8.0, 37°C, wild-type enzyme | Papaver somniferum | |
0.0508 | - |
(S)-Coclaurine | mutant G204E, pH 8.0, 30°C | Papaver somniferum | |
0.0624 | - |
(S)-Coclaurine | wild-type, pH 8.0, 30°C | Papaver somniferum | |
0.0624 | - |
(S)-Coclaurine | pH 8.0, 37°C, mutant enzyme G204E | Papaver somniferum | |
0.115 | - |
(S)-N-methylcoclaurine | pH 8.0, 37°C, wild-type enzyme | Papaver somniferum | |
0.115 | - |
(S)-N-methylcoclaurine | mutant G204E, pH 8.0, 30°C | Papaver somniferum | |
0.439 | - |
(S)-N-methylcoclaurine | wild-type, pH 8.0, 30°C | Papaver somniferum | |
0.439 | - |
(S)-N-methylcoclaurine | pH 8.0, 37°C, mutant enzyme G204E | Papaver somniferum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papaver somniferum | A0A1C9U5X5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + (S)-coclaurine | the mutant enzyme G204E produces (S)-N-methylcoclaurine and (S)-N,N-dimethylcoclaurine in a roughly 2:1 ratio | Papaver somniferum | 2 S-adenosyl-L-homocysteine + (S)-N,N-dimethylcoclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-coclaurine | reaction of EC 2.1.1.140 | Papaver somniferum | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-coclaurine | the mutant enzyme G204E produces (S)-N-methylcoclaurine and (S)-N,N-dimethylcoclaurine in a roughly 2:1 ratio | Papaver somniferum | S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-N-methylcoclaurine | - |
Papaver somniferum | S-adenosyl-L-homocysteine + (S)-N,N-dimethylcoclaurine | - |
? | |
S-adenosyl-L-methionine + (S)-N-methylcoclaurine | reaction of EC 2.1.1.140 | Papaver somniferum | S-adenosyl-L-homocysteine + ? | - |
? | |
S-adenosyl-L-methionine + (S)-reticuline | - |
Papaver somniferum | S-adenosyl-L-homocysteine + (S)-tembetarine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNMT | - |
Papaver somniferum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
(S)-Coclaurine | mutant G204E, pH 8.0, 30°C | Papaver somniferum | |
0.0000404 | - |
(S)-Coclaurine | pH 8.0, 37°C, wild-type enzyme | Papaver somniferum | |
0.000298 | - |
(S)-N-methylcoclaurine | pH 8.0, 37°C, mutant enzyme G204E | Papaver somniferum | |
0.0003 | - |
(S)-N-methylcoclaurine | wild-type, pH 8.0, 30°C | Papaver somniferum | |
0.0003 | - |
(S)-Coclaurine | wild-type, pH 8.0, 30°C | Papaver somniferum | |
0.000331 | - |
(S)-Coclaurine | pH 8.0, 37°C, mutant enzyme G204E | Papaver somniferum | |
0.0011 | - |
(S)-N-methylcoclaurine | mutant G204E, pH 8.0, 30°C | Papaver somniferum | |
0.00113 | - |
(S)-N-methylcoclaurine | pH 8.0, 37°C, wild-type enzyme | Papaver somniferum |