EC Number   |
Protein Variants |
Reference |
|---|
   2.1.1.244 | more |
depleting NRMT in 293LT cells, using lentivirus, significantly decreases methylation of endogenous RCC1, while not affecting overall RCC1 level, depleting NRMT in HeLa cells using short interfering RNAs causing the same effects, RCC1 methylation is rescued by expression of murine NRMT-FLAG, which is not targeted by the human shRNA |
718160 |
| 2.1.1.244 | more |
generation of NTMT1 knockout HEK-293FT cells by CRISPR-Cas9 |
756441 |
| 2.1.1.244 | more |
NRMT1 co-expression with NRMT2 increases the trimethylation rate of NRMT1. Generation of truncation constructs of NRMT1. Full-length FLAG-tagged NRMT1 only weakly interacts with the first 112 aa of NRMT2, though strongly interacts with a GFP-tagged NRMT2 fragment (amino acids 77-223) that is missing the 59 aa tail that is also lacking from the crystal structure and subsequent model. Full-length FLAG-tagged NRMT2 strongly interacts with the first 59 aa of NRMT1, as well as, amino acids 52-172. There is a decreased interaction of FLAG-tagged NRMT2-FLAG with the C-terminal fragment of NRMT1 (amino acids 178-223). Generation of NRMT1 knockout mutant, NRMT2 overexpression cannot rescue NRMT1 knockout phenotypes |
758296 |
| 2.1.1.244 | W136F |
site-directed mutagenesis, almost inactive mutant |
736186 |
| 2.1.1.244 | D180K |
site-directed mutagenesis, inactive mutant |
736186 |
| 2.1.1.244 | D180Y |
site-directed mutagenesis, inactive mutant |
736186 |
| 2.1.1.244 | H140K |
site-directed mutagenesis, inactive mutant |
736186 |
| 2.1.1.244 | Q169K |
site-directed mutagenesis, inactive mutant |
718160 |
| 2.1.1.244 | W136I |
site-directed mutagenesis, inactive mutant |
736186 |
| 2.1.1.244 | D178A/D181A |
site-directed mutagenesis, mutating the residues Asp178 and Asp181 at the lip of the active site to Ala decreases enzyme activity, which is further decreased by reverse-charge mutagenesis to Lys |
718160 |
