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Literature summary for 2.1.1.244 extracted from

  • Jia, K.; Huang, G.; Wu, W.; Shrestha, R.; Wu, B.; Xiong, Y.; Li, P.
    In vivo methylation of OLA1 revealed by activity-based target profiling of NTMT1 (2019), Chem. Sci., 10, 8094-8099 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information generation of NTMT1 knockout HEK-293FT cells by CRISPR-Cas9 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics Homo sapiens
0.00038
-
S-adenosyl-L-methionine with RCC1, pH and temperature not specified in the publication Homo sapiens
0.0014
-
(E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine with RCC1, pH and temperature not specified in the publication Homo sapiens
0.00196
-
N-terminal-[RCC1] with S-adenosyl-L-methionine, pH and temperature not specified in the publication Homo sapiens
0.00209
-
N-terminal-[RCC1] with (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine, pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + N-terminal-OLA1 Homo sapiens i.e. Obg-like ATPase 1 (OLA1) protein, target validation using normal and NTMT1 knockout HEK-293FT cells demonstrates that OLA1, a protein involved in many critical cellular functions, is methylated in vivo by NTMT1 3 S-adenosyl-L-homocysteine + ?
-
?
3 S-adenosyl-L-methionine + N-terminal-[RCC1] Homo sapiens
-
3 S-adenosyl-L-homocysteine + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BV86
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293FT cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine + N-terminal-OLA1
-
Homo sapiens ?
-
?
3 S-adenosyl-L-methionine + N-terminal-OLA1 i.e. Obg-like ATPase 1 (OLA1) protein, target validation using normal and NTMT1 knockout HEK-293FT cells demonstrates that OLA1, a protein involved in many critical cellular functions, is methylated in vivo by NTMT1 Homo sapiens 3 S-adenosyl-L-homocysteine + ?
-
?
3 S-adenosyl-L-methionine + N-terminal-OLA1 i.e. Obg-like ATPase 1 (OLA1) protein Homo sapiens 3 S-adenosyl-L-homocysteine + ?
-
?
3 S-adenosyl-L-methionine + N-terminal-[RCC1]
-
Homo sapiens 3 S-adenosyl-L-homocysteine + ?
-
?
3 S-adenosyl-L-methionine + N-terminal-[RCC1] RCC1p and methionine-removed RCC1 Homo sapiens 3 S-adenosyl-L-homocysteine + ?
-
?
additional information motif sequence and signal peptide analyses, and activity-based substrate profiling of NTMT1 utilizing (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine (Hey-SAM) reveals 72 potential targets, overview Homo sapiens ?
-
-

Synonyms

Synonyms Comment Organism
N-terminal methyltransferase 1
-
Homo sapiens
NTMT1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0067
-
(E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine with RCC1, pH and temperature not specified in the publication Homo sapiens
0.0075
-
S-adenosyl-L-methionine with RCC1, pH and temperature not specified in the publication Homo sapiens
0.0087
-
N-terminal-[RCC1] with (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine, pH and temperature not specified in the publication Homo sapiens
0.0097
-
N-terminal-[RCC1] with S-adenosyl-L-methionine, pH and temperature not specified in the publication Homo sapiens

Cofactor

Cofactor Comment Organism Structure
(E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine i.e. Hey-SAM Homo sapiens
S-adenosyl-L-methionine
-
Homo sapiens

General Information

General Information Comment Organism
malfunction aberrant N-terminal methylation has been implicated in several cancers and developmental diseases Homo sapiens
physiological function N-terminal methylation is a regulator of protein-DNA and protein-protein interactions for a number of proteins, such as RCC1, CENPA/B, DDB2, PARP3, an MYL9, playing important roles in cell mitotic progression, DNA damage repair, and regulation of protein function. N-terminal methyltransferase 1 (NTMT1) catalyzes the N-terminal methylation of proteins with a specific N-terminal motif after methionine removal. Obg-like ATPase 1 (OLA1) protein, a protein involved in many critical cellular functions, is methylated in vivo by NTMT1, NTMT1 is responsible for OLA1 methylation in vivo Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.16
-
N-terminal-[RCC1] with (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine, pH and temperature not specified in the publication Homo sapiens
4.79
-
(E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine with RCC1, pH and temperature not specified in the publication Homo sapiens
4.95
-
N-terminal-[RCC1] with S-adenosyl-L-methionine, pH and temperature not specified in the publication Homo sapiens
19.74
-
S-adenosyl-L-methionine with RCC1, pH and temperature not specified in the publication Homo sapiens