Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of NTMT1 knockout HEK-293FT cells by CRISPR-Cas9 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
0.00038 | - |
S-adenosyl-L-methionine | with RCC1, pH and temperature not specified in the publication | Homo sapiens | |
0.0014 | - |
(E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine | with RCC1, pH and temperature not specified in the publication | Homo sapiens | |
0.00196 | - |
N-terminal-[RCC1] | with S-adenosyl-L-methionine, pH and temperature not specified in the publication | Homo sapiens | |
0.00209 | - |
N-terminal-[RCC1] | with (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine, pH and temperature not specified in the publication | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine + N-terminal-OLA1 | Homo sapiens | i.e. Obg-like ATPase 1 (OLA1) protein, target validation using normal and NTMT1 knockout HEK-293FT cells demonstrates that OLA1, a protein involved in many critical cellular functions, is methylated in vivo by NTMT1 | 3 S-adenosyl-L-homocysteine + ? | - |
? | |
3 S-adenosyl-L-methionine + N-terminal-[RCC1] | Homo sapiens | - |
3 S-adenosyl-L-homocysteine + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BV86 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293FT cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine + N-terminal-OLA1 | - |
Homo sapiens | ? | - |
? | |
3 S-adenosyl-L-methionine + N-terminal-OLA1 | i.e. Obg-like ATPase 1 (OLA1) protein, target validation using normal and NTMT1 knockout HEK-293FT cells demonstrates that OLA1, a protein involved in many critical cellular functions, is methylated in vivo by NTMT1 | Homo sapiens | 3 S-adenosyl-L-homocysteine + ? | - |
? | |
3 S-adenosyl-L-methionine + N-terminal-OLA1 | i.e. Obg-like ATPase 1 (OLA1) protein | Homo sapiens | 3 S-adenosyl-L-homocysteine + ? | - |
? | |
3 S-adenosyl-L-methionine + N-terminal-[RCC1] | - |
Homo sapiens | 3 S-adenosyl-L-homocysteine + ? | - |
? | |
3 S-adenosyl-L-methionine + N-terminal-[RCC1] | RCC1p and methionine-removed RCC1 | Homo sapiens | 3 S-adenosyl-L-homocysteine + ? | - |
? | |
additional information | motif sequence and signal peptide analyses, and activity-based substrate profiling of NTMT1 utilizing (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine (Hey-SAM) reveals 72 potential targets, overview | Homo sapiens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
N-terminal methyltransferase 1 | - |
Homo sapiens |
NTMT1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
(E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine | with RCC1, pH and temperature not specified in the publication | Homo sapiens | |
0.0075 | - |
S-adenosyl-L-methionine | with RCC1, pH and temperature not specified in the publication | Homo sapiens | |
0.0087 | - |
N-terminal-[RCC1] | with (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine, pH and temperature not specified in the publication | Homo sapiens | |
0.0097 | - |
N-terminal-[RCC1] | with S-adenosyl-L-methionine, pH and temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
(E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine | i.e. Hey-SAM | Homo sapiens | |
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | aberrant N-terminal methylation has been implicated in several cancers and developmental diseases | Homo sapiens |
physiological function | N-terminal methylation is a regulator of protein-DNA and protein-protein interactions for a number of proteins, such as RCC1, CENPA/B, DDB2, PARP3, an MYL9, playing important roles in cell mitotic progression, DNA damage repair, and regulation of protein function. N-terminal methyltransferase 1 (NTMT1) catalyzes the N-terminal methylation of proteins with a specific N-terminal motif after methionine removal. Obg-like ATPase 1 (OLA1) protein, a protein involved in many critical cellular functions, is methylated in vivo by NTMT1, NTMT1 is responsible for OLA1 methylation in vivo | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.16 | - |
N-terminal-[RCC1] | with (E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine, pH and temperature not specified in the publication | Homo sapiens | |
4.79 | - |
(E)-hex-2-en-5-ynyl-S-adenosyl-L-methionine | with RCC1, pH and temperature not specified in the publication | Homo sapiens | |
4.95 | - |
N-terminal-[RCC1] | with S-adenosyl-L-methionine, pH and temperature not specified in the publication | Homo sapiens | |
19.74 | - |
S-adenosyl-L-methionine | with RCC1, pH and temperature not specified in the publication | Homo sapiens |