EC Number |
Protein Variants |
Reference |
---|
1.2.7.11 | K125A |
the mutant enzyme shows a large increase in the Km-value for CoA and shows poor inactivation by 4-fluoro-7-nitrobenzofurazan, compared with K173A and wild type enzyme |
718962 |
1.2.7.11 | K173A |
the mutant enzyme shows a large increase in the Km-value for CoA and shows poor inactivation by 4-fluoro-7-nitrobenzofurazan, compared with K173A and wild type enzyme |
718962 |
1.2.7.11 | I255L |
kcat/Km for pyruvate is 11% of wild-type value, kcat/KM for 2-oxoglutarate is 21% of wild-type value |
719399 |
1.2.7.11 | I255M |
kcat/Km for pyruvate is 13% of wild-type value, kcat/KM for 2-oxoglutarate is 2% of wild-type value |
719399 |
1.2.7.11 | I255S |
kcat/Km for pyruvate is 23% of wild-type value, kcat/KM for 2-oxoglutarate is 35% of wild-type value |
719399 |
1.2.7.11 | I255V |
kcat/Km for pyruvate is 14% of wild-type value, kcat/KM for 2-oxoglutarate is 38% of wild-type value |
719399 |
1.2.7.11 | P254G |
kcat/Km for pyruvate is 12% of wild-type value, kcat/KM for 2-oxoglutarate is 20% of wild-type value |
719399 |
1.2.7.11 | P257A |
no enzyme activity at either 50 or 80°C |
719399 |
1.2.7.11 | P257G |
no enzyme activity at either 50 or 80°C |
719399 |
1.2.7.11 | P257V |
no enzyme activity at either 50 or 80°C |
719399 |