EC Number   |
Protein Variants |
Reference |
|---|
   1.2.3.3 | more |
a cidC pyruvate oxidase mutant exhibited decreased pyruvate oxidase activity and shows reduced cell lysis in stationary phase |
-, 676050 |
| 1.2.3.3 | more |
enzyme deletion mutant, 20-25% decrease in acetate production during gr0wth on glucose as sole carbon source, 60-80% decrease in acetate production during growth on maltose, constitutive overproduction of enzyme, acetate becomes the only fermentation end product |
-, 655878 |
| 1.2.3.3 | more |
no pyruvate oxidase activity detectable in a PoxB/PoxF double mutant |
671480 |
| 1.2.3.3 | more |
poxB mutant, absence of pyruvate oxidase activity, which reduces glucose uptake rate, biomass yield, acetate production and cell growth rate, and increases O2 consumption and CO2 evolution. Glucokinase activity increases, whereas the flux through glycolysis is reduced due to down-regulation of other glycolytic enzymes such as 6-phosphofructosekinase and fructose bisphosphate aldolase. TCA cycle enzymes such as citrate synthase and malate dehydrogenase are repressed in the poxB mutant when cells are cultivated in LB medium. Activation of glucose-6-phosphate dehydrogenase and acetyl-CoA synthetase |
695267 |
| 1.2.3.3 | more |
SpxB-deficient mutant, shows drastically diminished H2O2 production |
-, 687414 |
| 1.2.3.3 | P178S/S188N/A458V |
stabilizes quaternary rather than tertiary structure |
390433 |
| 1.2.3.3 | V265A |
binds FAD more weakly |
654686 |
| 1.2.3.3 | V265A |
similar catalytic properties compared to the wild-type protein, but decreased affinity for FAD |
672023 |
