EC Number |
Protein Variants |
Reference |
---|
1.2.1.26 | A110S/K273A/A442P/P444T |
random mutagenesis, the mutant shows reduced activity with 3-hydroxypropionaldehyde and altered cofactor kinetics compared to the wild-type enzyme, no activity with NADP+ |
763713 |
1.2.1.26 | A110S/K273A/R334Q/A337R/A442P/P444T |
random mutagenesis, the mutant shows increased activity with 3-hydroxypropionaldehyde and altered cofactor kinetics compared to the wild-type enzyme |
763713 |
1.2.1.26 | A442P/P444T |
random mutagenesis, the mutant shows reduced activity with 3-hydroxypropionaldehyde and altered cofactor kinetics compared to the wild-type enzyme, no activity with NADP+ |
763713 |
1.2.1.26 | C287A |
site-directed mutagenesis, the mutant is almost inactive |
763717 |
1.2.1.26 | E253A |
site-directed mutagenesis, the mutant is almost inactive |
763717 |
1.2.1.26 | F156A |
site-directed mutagenesis, the mutant is almost inactive |
763717 |
1.2.1.26 | F450A |
site-directed mutagenesis, the mutant is almost inactive |
763717 |
1.2.1.26 | I288A |
site-directed mutagenesis, the mutant is almost inactive |
763717 |
1.2.1.26 | K273A/A442P/T443E/P444A |
random mutagenesis, the mutant shows increased activity with 3-hydroxypropionaldehyde and altered cofactor kinetics compared to the wild-type enzyme, no activity with NADP+ |
763713 |
1.2.1.26 | K273A/R334Q/A337R/A442P/T443E/P444A |
random mutagenesis, the mutant shows strongly increased activity with 3-hydroxypropionaldehyde and altered cofactor kinetics compared to the wild-type enzyme |
763713 |