Information on EC 1.2.1.26 - 2,5-dioxovalerate dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

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EC NUMBER
COMMENTARY hide
1.2.1.26
-
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RECOMMENDED NAME
GeneOntology No.
2,5-dioxovalerate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,5-dioxopentanoate + NADP+ + H2O = 2-oxoglutarate + NADPH + 2 H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
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reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-arabinose degradation III
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-
D-galactarate degradation II
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-
D-galacturonate degradation II
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-
D-glucarate degradation II
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-
D-glucuronate degradation II
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trans-4-hydroxy-L-proline degradation II
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xylose degradation III
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degradation of pentoses
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Pentose and glucuronate interconversions
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-
Ascorbate and aldarate metabolism
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-
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SYSTEMATIC NAME
IUBMB Comments
2,5-dioxopentanoate:NADP+ 5-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37250-92-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
show the reaction diagram
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
show the reaction diagram
acetaldehyde + NAD(P)+ + H2O
acetate + NAD(P)H + H+
show the reaction diagram
alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
alpha-ketoglutarate + NAD(P)H + H+
show the reaction diagram
alpha-ketoglutaric semialdehyde + NADP+ + H2O
alpha-ketoglutarate + NADPH + H+
show the reaction diagram
-
-
-
?
butyraldehyde + NAD(P)+ + H2O
n-butanoate + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
DL-glyceraldehyde + NADP+ + H2O
glycerate + NADPH + H+
show the reaction diagram
glutaraldehyde + NAD(P)+ + H2O
glutarate + NAD(P)H + H+
show the reaction diagram
glutaric semialdehyde + NADP+ + H2O
glutarate + NADPH
show the reaction diagram
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-
-
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glycolaldehyde + NADP+ + H2O
glycolate + NADPH + H+
show the reaction diagram
heptaldehyde + NAD(P)+ + H2O
n-heptanoate + NAD(P)H + H+
show the reaction diagram
-
-
-
-
?
hexylaldehyde + NAD(P)+ + H2O
n-hexanoate + NAD(P)H + H+
show the reaction diagram
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-
-
-
?
octylaldehyde + NAD(P)+ + H2O
n-octanoate + NAD(P)H + H+
show the reaction diagram
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-
-
-
?
propanal + NAD(P)+ + H2O
propionate + NAD(P)H + H+
show the reaction diagram
propionaldehyde + NAD(P)+ + H2O
propionate + NAD(P)H + H+
show the reaction diagram
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-
-
-
?
succinate semialdehyde + NAD(P)+ + H2O
? + NAD(P)H + H+
show the reaction diagram
valeraldehyde + NAD(P)+ + H2O
n-pentanoate + NAD(P)H + H+
show the reaction diagram
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-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,5-dioxopentanoate + NAD(P)+ + H2O
2-oxoglutarate + NAD(P)H
show the reaction diagram
2,5-dioxopentanoate + NADP+ + H2O
2-oxoglutarate + NADPH + 2 H+
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-Hydroxy-alpha-ketoglutaric semialdehyde
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-
additional information
no inhibitory effect with alpha-ketoglutarate or glutamate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-galactarate
induces KGSADH-II
D-glucarate
induces KGSADH-II
hydroxy-L-proline
induces KGSADH-III
L-arabinose
induces KGSADH-I
NADP+
-
the enzyme prefers NADP+ over NAD+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 0.1
2,5-dioxopentanoate
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-
3.9
acetaldehyde
with NADP+ as cofactor
0.0065 - 0.5569
alpha-Ketoglutaric semialdehyde
0.17
butylaldehyde
-
-
0.1 - 0.31
Glutaraldehyde
0.016
Glutaric semialdehyde
-
-
0.37
glycolaldehyde
-
pH 6.0, 70°C, native enzyme form xylose-grown cells
0.0393
heptaldehyde
-
-
0.0814
Hexylaldehyde
-
-
0.1169 - 3
NAD+
0.0149 - 2.427
NADP+
0.0352
Octylaldehyde
-
-
0.3 - 1.05
propanal
1.46
propionaldehyde
-
-
0.45 - 1.66
succinate semialdehyde
0.145
Valeraldehyde
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-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.6
2,5-dioxopentanoate
-
pH 7.5, 70°C
0.41
acetaldehyde
with NADP+ as cofactor
1.12 - 197
alpha-Ketoglutaric semialdehyde
1.35
butylaldehyde
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-
4.8
DL-glyceraldehyde
-
pH 7.5, 70°C
0.65 - 4.13
Glutaraldehyde
5.3
glycolaldehyde
-
pH 7.5, 70°C
0.87
heptaldehyde
-
-
1.47
Hexylaldehyde
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-
0.74 - 57
NAD+
1.08 - 55
NADP+
0.97
Octylaldehyde
-
-
0.58 - 2.07
propanal
1.29
propionaldehyde
-
-
0.05 - 0.06
succinate semialdehyde
2.1
Valeraldehyde
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
-
2,5-dioxopentanoate + NADP+
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
not active below, 2,5-dioxopentanoate + NADP+
6.7 - 8.2
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more than 50% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
12000
gel filtration; gel filtration
52290
-
4 * 52290, calculated from sequence
54351
2 * 57000 or 2 * 54351, gel filtration
55185
2 * 57000 or 2 * 55185, gel filtration
57000
2 * 57000 or 2 * 54351, gel filtration; 2 * 57000 or 2 * 55185, gel filtration
62000
2 * 62000, SDS-PAGE
115000
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sucrose density gradient centrifugation
210100
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mass spectrometry
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
tetramer
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
86
-
half-life time: 18 min
90
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half-life time: below 10 min
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-35°C, buffer E, pH 8.0, 250 mM imidazole
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by gel filtration
KGSADH-III purified on nickel-nitrilotriacetic acid spin column and by gel filtration; KGSADH-II purified on nickel-nitrilotriacetic acid spin column and by gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as His6-tagged ycbD protein
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expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
KGSADH-III overexpressed as His6-tagged protein in Escherichia coli DH5alpha; KGSADH-II overexpressed as His6-tagged protein in Escherichia coli DH5alpha
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
additional information
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Show AA Sequence (390 entries)
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LINKS TO OTHER DATABASES (specific for EC-Number 1.2.1.26)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)