EC Number   |
Protein Variants |
Reference  |
|---|
    1.2.1.13 | more |
construction of hybrid enzymes between the glyceraldehyde-3-phosphate dehydrogenases from the mesophilic Methanobacterium bryantii and the thermophilic Methanothermus fervidus |
287989 |
| 1.2.1.13 | Y123W |
increase of temperature of irreversible inactivation by 1.3°C |
287989 |
| 1.2.1.13 | Y323S |
decrease of temperature of irreversible inactivation by 4.5°C |
287989 |
| 1.2.1.13 | K128A |
Km-value for NADPH is nearly identical to wild-type value, turnover-number for NADPH is decreased about 2fold. Km-value for NADH is increased 2.1fold compared to wild-type enzyme, turnover-number for NADH is decreased 2.6fold |
655492 |
| 1.2.1.13 | K128E |
Km-value for NADPH is about 80% of the wild-type value, turnover-number for NADPH is decreased 2.7fold. Km-value for NADH is increased 2.3fold compared to wild-type enzyme, turnover-number for NADH is decreased 1.9fold |
655492 |
| 1.2.1.13 | R197A |
Km-value for NADPH is identical to wild-type value, turnover-number for NADPH is about 90% of the wild-type value. Km-value for NADH is about 80% of the wild-type value, turnover-number for NADH is nearly identical to wild-type value |
655492 |
| 1.2.1.13 | R197E |
Km-value for NADPH is increased 1.3fold compared to wild-type enzyme, turnover-number for NADPH is decreased 2fold. Km-value for NADH is about 90% of the wild-type value, turnover-number for NADH is increased 1.3fold |
655492 |
| 1.2.1.13 | C18S |
mutant of GapB subunit still shows stron redox regulation |
656096 |
| 1.2.1.13 | C274S |
mutant of GapB subunit still shows stron redox regulation |
656096 |
| 1.2.1.13 | C285S |
mutant of GapB subunit still shows stron redox regulation |
656096 |
