EC Number |
Protein Variants |
Reference |
---|
1.14.14.11 | L44A |
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene |
-, 714587 |
1.14.14.11 | L45A |
mutation leads to a change of substrate preference. The mutant enzyme exhibits a remarkably reversed substrate preference compared to the wild type, displaying increased reactivity with an increase in substrate size. The activity of L45A is about 5% of that of the wild type towards styrene, but reaches about 30 and about 90% when the substrate is changed to 2-methylstyrene and 2-ethylstyrene, respectively |
-, 714587 |
1.14.14.11 | more |
construcution of fusion proteins that join the C-terminus of the epoxidase StyA to the N-terminus of FAD reductase StyB through a linker peptide and application in the synthesis of of a broad range of substituted indoles to indigoid chromophores. The fusion proteins are self-regulated and couple efficiently NADH oxidation to styrene epoxidation |
744072 |
1.14.14.11 | more |
the random mutation of styrene monooxygenase using error prone PCR generates a number of improved variants exhibiting the highest known rate of (S)-styrene epoxide and (1S-2R) indene epoxide production by a biocatalyst |
-, 713866 |
1.14.14.11 | N46A |
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene |
-, 714587 |
1.14.14.11 | R43A |
mutation leads to a change of substrate preference, substrate preference for 2-methylstyrene |
-, 714587 |
1.14.14.11 | T200V |
mutant exhibits lower reactivity than the wild type, displayed impaired diastereoselectivity |
714725 |
1.14.14.11 | Y73F |
mutant exhibits lower reactivity than the wild type |
714725 |