EC Number   |
Protein Variants |
Reference |
|---|
   1.1.1.202 | D41A |
mutation for relaxation of the coenzyme specificity, weakens the repulsion between Asp41 and the phosphate group esterified to the 2-hydroxyl group of the ribose at the adenine end of NADPH |
712531 |
| 1.1.1.202 | D41G |
mutation for relaxation of the coenzyme specificity, weakens the repulsion between Asp41 and the phosphate group esterified to the 2-hydroxyl group of the ribose at the adenine end of NADPH |
712531 |
| 1.1.1.202 | more |
engineering of a strain for optimized elimination of 3-hydroxypropionaldehyde, an inhibitory intermediary metabolite in the 1,3-propanediol synthesis pathway, from the broth during fermentation process of raw material required for the synthesis of polytrimethylene terephthalate and other polyester fibers, overview |
-, 688171 |
| 1.1.1.202 | more |
Klebsiella pneumoniae AK mutant strain defective in 1,3-PD oxidoreductase activity (DhaT). The Escherichia coli yqhD homolog can replace the function of DhaT. The yqhD homolog restores propane-1,3-diol production and 1,3-PD oxidoreductase activity in the mutant Klebsiella pneumoniae strain defective in DhaT. Level of propane-1,3-diol production during batch fermentation in the recombinant strain is comparable to that of the parent strain |
695844 |
| 1.1.1.202 | more |
Klebsiella pneumoniae mutant strain AK (dhaD and dhaK replaced with the lacZ promoter-apramycin resistance gene cassette, such that the lacZ promoter is inserted in front of dhaB) and mutant strain AR (deletion of the dhaR gene and replaced with the lacZ promoter-apramycin resistance gene cassette, defective in the oxidative branch and in the reductive branch due to the loss of the dhaT and orfWX). propane-1,3-diol is still produced at lower level in the mutant strains, even though dhaT is removed, thus a a putative oxidoreductase may catalyze the production of propane-1,3-diol at lower level compared to DhaT. Dramatic increase in the production of propane-1,3-diol in the recombinant strains harboring the plasmid pBR-dhaT or pBR-dhaT-orfWX, which contain dhaT. Amount of propane-1,3-diol produced is lower in the AK and AR strain, which is harboring pBR-dhaT-orfWX (5.2 g/l), than the corresponding Cu, which is harboring pBR-dhaT-orfWX (7.7 g/l). During fermentation, the production yield is higher in the AK strain (0.57 mol/mol) than the Cu strain (0.47 mol/mol) harboring pBR-dhaT-orfWX |
-, 695842 |
| 1.1.1.202 | more |
the PDOR isozyme activity increases 4.6times, when the yqhD gene is expressed in the Klebsiella pneumoniae mutant strains AK, which is a knockout mutant of the GDH and PDOR |
-, 740214 |
| 1.1.1.202 | nore |
expression of the Clostridumm butyricum enzyme in Clostridium acetobutylicum strain DG1(pSPD5) leads to functional reduction of glycerol to 1,3-propanediol in a two-step process, the first step of glycerol reduction to 3-hydroxypropionaldehyde, which is then reduced to 1,3-propanediol, is performed by the B12-independent glycerol dehydratase |
-, 667270 |
