EC Number   |
Protein Variants |
Reference  |
|---|
    6.3.5.7 | D178E |
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable |
662450 |
| 6.3.5.7 | D178N |
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable |
662450 |
| 6.3.5.7 | K254E |
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable |
662450 |
| 6.3.5.7 | T101A |
glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable |
662450 |
| 6.3.5.7 | T101S |
hydrolyzes about 10% of glutamine compared to wild-type enzyme. Compared to wild-type enzyme, the mutant enzyme converts approximately half as much mischarged tRNA substrate to product |
662450 |
| 6.3.5.7 | T177S |
mutant enzyme hydrolyzes the same amount of glutamine as the wild-type enzyme. As the wild-type enzyme, the mutant enzyme transforms most of Glu-tRNAGln to Gln-tRNAGln |
662450 |
| 6.3.5.7 | T177V |
glutamine hydrolysis is negligible. Gln-tRNAGln formation is undetectable |
662450 |
| 6.3.5.7 | S128T |
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln |
674787 |
| 6.3.5.7 | S152A |
mutant is glutaminase inactive |
674787 |
| 6.3.5.7 | S152T |
mutant is glutaminase inactive |
674787 |
