EC Number   |
Protein Variants |
Reference  |
|---|
   2.1.1.56 | more |
construction of carboxyl-truncated D1 genes, amino acid 1-666 and 1-425: 347 residues are sufficient for activity |
485455 |
| 2.1.1.56 | more |
mapping of lipid-binding site, membrane binding activity of wild-type and diverse constructed mutants |
485465 |
| 2.1.1.56 | more |
exchange for alanine at single sites within the methyltransferase domain fragment of D1, loss of activity when exchanged hits His682 and Tyr683, active site |
485468 |
| 2.1.1.56 | G600A |
in D1 fragment, amino acids 498-844, 4% activity remaining |
485472 |
| 2.1.1.56 | H682A |
in D1 fragment, amino acids 498-844, 4% activity remaining, unable to crosslink the cap in presence of S-adenosyl-L-homocysteine |
485472 |
| 2.1.1.56 | more |
exchange of 8 residues for alanine in the D1 fragment, amino acids 498-844 |
485472 |
| 2.1.1.56 | Y683A |
in D1 fragment, amino acids 498-844, 0.05% activity remaining, unable to crosslink the cap in presence of S-adenosyl-L-homocysteine |
485472 |
| 2.1.1.56 | Y683F |
in D1 fragment, amino acids 498-844, 4% activity remaining |
485472 |
| 2.1.1.56 | Y683S |
in D1 fragment, amino acids 498-844, catalytically defective, unable to crosslink the cap in presence of S-adenosyl-L-homocysteine |
485472 |
| 2.1.1.56 | D122A/S123A |
site-directed mutagenesis, complementation of the yeast abd1DELTA mutant at 25°C and 30°C, only slightly at 37°C |
659921 |
