EC Number |
Protein Variants |
Reference |
---|
1.5.5.2 | Y540A |
a construct consisting of residue 86-630 of PutA is used |
696361 |
1.5.5.2 | Y540S |
a construct consisting of residue 86-630 of PutA is used |
696361 |
1.5.5.2 | F10E/L12E |
construction of a variant with a more polar N-terminus, mutant F10E/L12E, because Thermus thermophilus ProDH (TtProDH), produced through fusion with maltose-binding protein (MBP) appears to be prone to aggregation. Preparation of TtProDH mutant variant DELTAABC, which lacks helices alphaA, alphaB and alphaC, the mutant shows no electron density for an AMP moiety of the cofactor |
-, 743834 |
1.5.5.2 | more |
expression of PRODH/POX in DLD-1 colorectal cancer cells significantly decreases basal and maximal respiration at both 3 and 5 days, and proline addition exacerbates this effect. PRODH/POX-dependent inhibition of respiration can be modulated by the duration of PRODH/POX expression and the availability of proline |
741588 |
1.5.5.2 | more |
generation of an enzyme-deficient prodh1 mutant |
741858 |
1.5.5.2 | K110A |
inactive |
724389 |
1.5.5.2 | D370A/Y540F |
mutant form of PutA86-601. The ratio of turnover-number to Km-value is 15fold lower than the ratio of the wild-type PutA86-601 |
658211 |
1.5.5.2 | D370A |
mutant form of PutA86-601. The ratio of turnover-number to Km-value is 2.8fold lower than the ratio of the wild-type PutA86-601 |
658211 |
1.5.5.2 | Y540F |
mutant form of PutA86-601. The ratio of turnover-number to Km-value is 5.8fold lower than the ratio of the wild-type PutA86-601 |
658211 |
1.5.5.2 | L432P |
mutant form of PutA86-669, 5fold decrease in turnover-number and a severe loss in thermostability |
658033 |