EC Number |
Protein Variants |
Reference |
---|
1.5.5.2 | more |
mutants deficient in proline dehydrogenase activity |
392572 |
1.5.5.2 | L432P |
mutant form of PutA86-669, 5fold decrease in turnover-number and a severe loss in thermostability |
658033 |
1.5.5.2 | D370A |
mutant form of PutA86-601. The ratio of turnover-number to Km-value is 2.8fold lower than the ratio of the wild-type PutA86-601 |
658211 |
1.5.5.2 | D370A/Y540F |
mutant form of PutA86-601. The ratio of turnover-number to Km-value is 15fold lower than the ratio of the wild-type PutA86-601 |
658211 |
1.5.5.2 | Y540F |
mutant form of PutA86-601. The ratio of turnover-number to Km-value is 5.8fold lower than the ratio of the wild-type PutA86-601 |
658211 |
1.5.5.2 | more |
truncated enzyme containing residues 86-601 and only four Trp residues. Substantial conformational changes of truncated protein upon addition of proline |
671977 |
1.5.5.2 | W194F |
mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 50% |
671977 |
1.5.5.2 | W194F/W211F |
mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 95% |
671977 |
1.5.5.2 | W211F |
mutation in truncated enzyme containing residues 86-601, ratio of kcat to Km-value drops by 80% |
671977 |
1.5.5.2 | Y540A |
a construct consisting of residue 86-630 of PutA is used |
696361 |