EC Number   |
Protein Variants |
Reference |
|---|
    5.4.99.39 | F413V |
site-directed mutagenesis, functional analysis, the mutant shows slightly reduced activity compared to thre wild-type enzyme |
748812 |
| 5.4.99.39 | F413W |
site-directed mutagenesis, functional analysis, the mutant shows slightly increased activity compared to thre wild-type enzyme |
748812 |
| 5.4.99.39 | F413Y |
site-directed mutagenesis, functional analysis, the mutant shows slightly reduced activity compared to thre wild-type enzyme |
748812 |
| 5.4.99.39 | F474A |
site-directed mutagenesis, the mutant produces significantly larger amounts of the bicyclic products and a decreased amount of beta-amyrin compared to wild-type. The mutant variant produces (9betaH)-polypoda-7,13,17,21-tetraen-3beta-ol and (9betaH)-polypoda-8(26),13,17,21-tetraen-3beta-ol, which are generated from a chair-boat folding conformation |
748805 |
| 5.4.99.39 | F474G |
site-directed mutagenesis, the mutant produces significantly larger amounts of the bicyclic products and a decreased amount of beta-amyrin compared to wild-type |
748805 |
| 5.4.99.39 | F474H |
site-directed mutagenesis |
748805 |
| 5.4.99.39 | F474L |
site-directed mutagenesis, the mutant shows a significantly decreased production of bicyclic compounds, and in turn exhibits a higher production of beta-amyrin compared to wild-type |
748805 |
| 5.4.99.39 | F474M |
site-directed mutagenesis, the mutant shows a significantly decreased production of bicyclic compounds, and in turn exhibits a higher production of beta-amyrin compared to wild-type |
748805 |
| 5.4.99.39 | F474T |
site-directed mutagenesis |
748805 |
| 5.4.99.39 | F474V |
site-directed mutagenesis, the mutant shows a significantly decreased production of bicyclic compounds, and in turn exhibits a higher production of beta-amyrin compared to wild-type |
748805 |
