EC Number   |
Protein Variants |
Reference |
|---|
    5.4.99.39 | Y259F |
site-directed mutagenesis, functional analysis |
748812 |
| 5.4.99.39 | Y259H |
site-directed mutagenesis, functional analysis |
748812 |
| 5.4.99.39 | Y259L |
site-directed mutagenesis, functional analysis |
748812 |
| 5.4.99.39 | Y259V |
site-directed mutagenesis, functional analysis |
748812 |
| 5.4.99.39 | Y259W |
site-directed mutagenesis, functional analysis |
748812 |
| 5.4.99.39 | Y261H |
mutation in region responsible for product differentiation. Mutant produces 2.4% lupeol, 13.6% germanicol and 84% of dammara-18(E),21-dien-3beta-ol, dammara-18(Z),21-dien-3beta-ol and dammara-18(28),21-dien-3beta-ol |
683581 |
| 5.4.99.39 | Y560F |
site-directed mutagenesis, the mutation of the key residue of GsAS2 results in 71.15% reduced catalytic efficiency compared to wild-type GsAS2 |
749323 |
| 5.4.99.39 | Y560H |
site-directed mutagenesis, the mutation of the key residue of GsAS2 results in 41.3% reduced catalytic efficiency compared to wild-type GsAS2 |
749323 |
