EC Number   |
Protein Variants |
Reference |
|---|
    4.2.1.51 | F185Y |
constructed by PCR-based random mutagenesis and complementation analysis, 96% reduced activity compared to the wild-type enzyme, 26% increased Km |
649516 |
| 4.2.1.51 | H209A |
site-directed mutagenesis, highly reduced activity |
649980 |
| 4.2.1.51 | more |
alterations of Asp76, Glu89, His115, and Arg236 do not cause significant changes in the kinetics properties |
-, 649516 |
| 4.2.1.51 | more |
expression of genes contaninig P-protein domains or subdomains, comparison of their catalytic activities and activation by phenylalanine |
3570 |
| 4.2.1.51 | more |
production of inhibitor-insensitive mutants |
33895 |
| 4.2.1.51 | N160A |
site-directed mutagenesis, 500fold decreased activity |
649980 |
| 4.2.1.51 | N160D |
site-directed mutagenesis, highly reduced activity |
649980 |
| 4.2.1.51 | Q215A |
site-directed mutagenesis, 500fold decreased activity |
649980 |
| 4.2.1.51 | R184L |
constructed by PCR-based random mutagenesis and complementation analysis, 50% reduced activity compared to the wild-type enzyme |
649516 |
| 4.2.1.51 | S208A |
site-directed mutagenesis, 100fold decreased activity |
649980 |
