4.2.1.51	C216A	site-directed mutagenesis, inactive mutant	649980	
4.2.1.51	C216S	site-directed mutagenesis, increased activity	649980	
4.2.1.51	E159A	site-directed mutagenesis, 2.2fold increased activity	649980	
4.2.1.51	E159A/E232A	site-directed mutagenesis, 7fold increased kcat, 4.6fold decreased Km compared to the wild-type, increased activity	649980	
4.2.1.51	E232A	site-directed mutagenesis, 3.5fold increased activity	649980	
4.2.1.51	E64D	constructed by PCR-based random mutagenesis and complementation analysis, 15% reduced activity compared to the wild-type enzyme, 4.5% increased Km, 1.7fold increased kcat	649516	
4.2.1.51	E64Q	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	E64S	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	E64V	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	F185L	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	F185Y	constructed by PCR-based random mutagenesis and complementation analysis, 96% reduced activity compared to the wild-type enzyme, 26% increased Km	649516	
4.2.1.51	H209A	site-directed mutagenesis, highly reduced activity	649980	
4.2.1.51	additional information	alterations of Asp76, Glu89, His115, and Arg236 do not cause significant changes in the kinetics properties	-, 649516	
4.2.1.51	additional information	expression of genes contaninig P-protein domains or subdomains, comparison of their catalytic activities and activation by phenylalanine	3570	
4.2.1.51	additional information	production of inhibitor-insensitive mutants	33895	
4.2.1.51	N160A	site-directed mutagenesis, 500fold decreased activity	649980	
4.2.1.51	N160D	site-directed mutagenesis, highly reduced activity	649980	
4.2.1.51	Q215A	site-directed mutagenesis, 500fold decreased activity	649980	
4.2.1.51	R184L	constructed by PCR-based random mutagenesis and complementation analysis, 50% reduced activity compared to the wild-type enzyme	649516	
4.2.1.51	S208A	site-directed mutagenesis, 100fold decreased activity	649980	
4.2.1.51	S208C	site-directed mutagenesis, 100fold decreased activity	649980	
4.2.1.51	S208D	site-directed mutagenesis, inactive mutant	649980	
4.2.1.51	S298I	mutant Mtr1	694629	
4.2.1.51	S99A	constructed by PCR-based random mutagenesis and complementation analysis, insensitive to inhibition by phenylalanine	-, 649516	
4.2.1.51	S99C	constructed by PCR-based random mutagenesis and complementation analysis, insensitive to inhibition by phenylalanine	-, 649516	
4.2.1.51	S99L	constructed by PCR-based random mutagenesis and complementation analysis, insensitive to inhibition by phenylalanine	-, 649516	
4.2.1.51	S99M	constructed by PCR-based random mutagenesis and complementation analysis, 30% reduced activity compared to the wild-type enzyme, insensitive to inhibition by phenylalanine	-, 649516	
4.2.1.51	T183A	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	T183S	constructed by PCR-based random mutagenesis and complementation analysis, highly reduced activity	649516	
4.2.1.51	T183Y	constructed by PCR-based random mutagenesis and complementation analysis, inactive mutant	649516	
4.2.1.51	T278A	site-directed mutagenesis, catalytically inactive mutant, but binds to substrate and inhibitor	649980	
4.2.1.51	T278S	site-directed mutagenesis, 100fold decreased activity	649980	
4.2.1.51	T278V	site-directed mutagenesis, catalytically inactive mutant, but binds to substrate and inhibitor	649980	
4.2.1.51	T326P	mutant harboring pheAfbr retains more than 70% of CM and PDT activities even in the presence of 200 mM L-phenylalanine, has potential in overproduction of L-phenylalanine	-, 702720	
4.2.1.51	W226A	site-directed mutagenesis, nearly inactive mutant	649980	
4.2.1.51	W226L	site-directed mutagenesis, highly reduced activity	649980