EC Number |
Protein Variants |
Reference |
---|
2.7.1.29 | H9A |
completely inactive |
641271 |
2.7.1.29 | K382A |
the mutant shows activity with polyphosphate |
-, 738418 |
2.7.1.29 | K514A |
the mutant shows activity with polyphosphate |
-, 738418 |
2.7.1.29 | more |
1-propanol production from glycerol is achieved by addition of the ATP-dependent dihydroxyacetone kinase gene to Escherichia coli strain BW38029 harboring pKK_mde and pRSF_pduCDEGOQS, reconstruction of the 1,2-propanediol (1,2-PD) synthetic pathway (pKK_mde), pathway overview |
-, 759519 |
2.7.1.29 | more |
by using the power of CRISPR spacers to link viruses to their prokaryotic hosts, the virus-host interactions in geographically diverse salterns are explored. Metagenomic CRISPRs detected with two independent methods map haloviruses to saltern hosts |
-, 759901 |
2.7.1.29 | more |
constitutive co-overexpression of gene gldA1 or dhaD1, encoding a glycerol dehydrogenase (Gldh), and gene dhaK, encoding dihydroxyacetone kinase, as a fused protein results in a significant payoff in cell growth and acetone-butanol-ethanol (ABE) production compared to expression of one Gldh. Overexpression of [(dhaD1 + gldA1) dhaK] improves butanol and ABE production by 70% and 50%, respectively, in the presence of 5 and 6 g/l furfural relative to the plasmid control. Constitutive overexpression of two Gldh [dhaD1 + gldA1] as a fused protein increases glycerol utilization by 43% relative to the plasmid control, thus, representing about 14% increase in glycerol consumption relative to overexpression of GldA1 or DhaD1 alone. With [(dhaD1 + gldA1) dhaK], 28.6% increase in glycerol utilization is observed (compared to 43% by dhaD1 + gldA1), relative to the plasmid control. Inability of recombinant Clostridium beijerinckii to metabolize glycerol as a sole substrate. Method development and evaluation, overview |
-, 758952 |
2.7.1.29 | R207B |
the mutant shows activity with polyphosphate |
-, 738418 |
2.7.1.29 | R475A |
the mutant shows activity with polyphosphate |
-, 738418 |