EC Number   |
Protein Variants |
Reference |
|---|
   2.4.1.65 | K73T/R110W/E111D/V112I |
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme |
693000 |
| 2.4.1.65 | K97S |
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity |
693000 |
| 2.4.1.65 | more |
construction of several chimeric mutants by domain swappig, overview |
693000 |
| 2.4.1.65 | more |
with full length UA948FucT, only 20% of total enzyme activities are localized in the soluble fraction. For UA948(1-428), this increases to 47%, respectively, indicating that truncation of the C-terminal putative alpha-helices increases FucT solubility. UA948(1-364), a mutant with the entire heptad repeat region removed, exhibits extremely low levels of enzyme activity. This indicates that the heptad repeat region is essential for enzyme activity. Construct UA948(1-371) has little enzyme activity. UA948(10-434) and UA948(1-434) completely lose activity |
-, 680736 |
| 2.4.1.65 | R110H |
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity |
661839 |
| 2.4.1.65 | R110K |
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity |
661839 |
| 2.4.1.65 | R110N |
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity |
661839 |
| 2.4.1.65 | R110Q |
less than 10% of the wild type alpha-1,3-activity and undetectable alpha-1,4-activity |
661839 |
| 2.4.1.65 | R110Q |
naturally occurring missense mutation in the FUT7 gene, the mutation abolishes FUT7 enzyme activity and synthesis of E-selectin binding ligands on leukocytes, and increases the expression of FUT4, the mutant shows unaltered synthesis of P-selectin binding ligand, overview |
662567 |
| 2.4.1.65 | R110W |
no alpha-1,3-activity or alpha-1,4-activity |
661839 |
