EC Number   |
Protein Variants |
Reference |
|---|
   1.2.1.48 | H411Y |
disruption of the key abstraction of the hydroxyl hydrogen |
693389 |
| 1.2.1.48 | K449E |
the mutant shows slight reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme |
762884 |
| 1.2.1.48 | N112A |
site-directed mutagenesis, inactive mutant |
743336 |
| 1.2.1.48 | Q445R |
substitution in the recombinant protein |
693389 |
| 1.2.1.48 | T184M |
disruption of NAD+ binding/utilisation |
693389 |
| 1.2.1.48 | T184R |
disruption of NAD+ binding/utilisation |
693389 |
| 1.2.1.48 | Y113F |
site-directed mutagenesis, the mutant activity is unaltered compared to wild-type |
743336 |
| 1.2.1.48 | Y410F |
site-directed mutagenesis, the mutant shows normal Vmax/KM levels against octanal and dodecanal and a somewhat reduced but still considerable catalytic capacity for hexadecanal |
743336 |
| 1.2.1.48 | Y92A |
the mutant shows slight reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme |
762884 |
