1.2.1.48	C241S	site-directed mutagenesis, inactive active-site mutant	743336	
1.2.1.48	C244S	the mutant shows about 90% reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	D245N	NAD+ cosubstrate binding is occuring but catalytic reduction is diminished	693389	
1.2.1.48	E207Q	site-directed mutagenesis, inactive mutant	743336	
1.2.1.48	E331Q	site-directed mutagenesis, inactive mutant	743336	
1.2.1.48	F456A	the mutant shows about 50% reduction in catalytic efficiency with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	F456A/F457A	the mutant shows about 15% reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	F457A	the mutant shows about 40% reduction in catalytic efficiency with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	G185A	disruption of NAD+ binding/utilisation	693389	
1.2.1.48	G412R	disruption of interaction of side-chains with substrate	693389	
1.2.1.48	H411Y	disruption of the key abstraction of the hydroxyl hydrogen	693389	
1.2.1.48	K449E	the mutant shows slight reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme	762884	
1.2.1.48	N112A	site-directed mutagenesis, inactive mutant	743336	
1.2.1.48	Q445R	substitution in the recombinant protein	693389	
1.2.1.48	T184M	disruption of NAD+ binding/utilisation	693389	
1.2.1.48	T184R	disruption of NAD+ binding/utilisation	693389	
1.2.1.48	Y113F	site-directed mutagenesis, the mutant activity is unaltered compared to wild-type	743336	
1.2.1.48	Y410F	site-directed mutagenesis, the mutant shows normal Vmax/KM levels against octanal and dodecanal and a somewhat reduced but still considerable catalytic capacity for hexadecanal	743336	
1.2.1.48	Y92A	the mutant shows slight reduction in catalytic efficiency with benzaldehyde and no activity with 4,4'-diapolycopendial compared to the wild type enzyme	762884