EC Number   |
Protein Variants |
Reference  |
|---|
   1.14.20.5 | L311F/F146I/A120M |
the activity of the triple mutant falls to about 5% of the wild type enzyme |
742512 |
| 1.14.20.5 | L311F/F146I/Y240P |
inactive mutant |
742512 |
| 1.14.20.5 | L311F/F146I/Y240P/A120M |
inactive mutant |
742512 |
| 1.14.20.5 | L311F/Y240P |
the double mutant exhibits less flavone synthase activity than either the wild type or the Y240P single mutant enzymes |
742512 |
| 1.14.20.5 | Y240P |
the mutant enzyme converts naringenin to apigenin without producing any 2-hydroxynaringenin. The mutant has a higher affinity (lower Km) with naringenin than the wild type enzyme, but the catalytic efficiency of the mutant is lower than that of wild type enzyme. The mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 104% of the catalytic activity relative to wild-type enzyme |
742512 |
| 1.14.20.5 | Y240P/A120M |
the enzyme activity of the double mutant is reduced to a level of about 10% of the activity of the wild type enzyme and the only product generated is apigenin. The mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 175% of the catalytic activity relative to wild-type enzyme |
742512 |
| 1.14.20.5 | Y240P/F146I |
the enzyme activity of the double mutant is reduced to a level of about 10% of the activity of the wild type enzyme and the only product generated is apigenin |
742512 |
