EC Number   |
Protein Variants |
Reference |
|---|
   1.14.20.5 | Y240P/A120M |
the enzyme activity of the double mutant is reduced to a level of about 10% of the activity of the wild type enzyme and the only product generated is apigenin. The mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 175% of the catalytic activity relative to wild-type enzyme |
742512 |
| 1.14.20.5 | Y240P |
the mutant enzyme converts naringenin to apigenin without producing any 2-hydroxynaringenin. The mutant has a higher affinity (lower Km) with naringenin than the wild type enzyme, but the catalytic efficiency of the mutant is lower than that of wild type enzyme. The mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 104% of the catalytic activity relative to wild-type enzyme |
742512 |
| 1.14.20.5 | more |
transgenic seedlings of Arabidopsis thaliana accumulate substantial amounts of apigenin, and the apigenin level correlates with the abundance of enzyme mRNA |
685694 |
| 1.14.20.5 | more |
use of enzyme for construction of a system for producing unnatural flavonoids and stilbenes in Escherichia coli by expression of the respective genes on three plasmids. Incubation of the recombinant Escherichia coli with exogenously supplied carboxylic acids leads to production of 87 different polyketides, including 36 unnatural flavonoids and stilbenes |
679173 |
| 1.14.20.5 | A120M |
when the purified recombinant mutant enzyme is incubated with naringenin as substrate, the ratio of 2-hydroxynaringenin to apigenin is reduced. The mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 162% of the catalytic activity relative to wild-type enzyme |
742512 |
| 1.14.20.5 | L311F |
when the purified recombinant mutant enzyme is incubated with naringenin as substrate, the ratio of 2-hydroxynaringenin to apigenin is reduced. The mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 355% of the catalytic activity relative to wild-type enzyme |
742512 |
| 1.14.20.5 | F146I |
when the purified recombinant mutant enzyme is incubated with naringenin as substrate, the ratio of 2-hydroxynaringenin to apigenin is reduced. The mutant enzyme is able to oxidize dihydrokaempferol to kaempferol with 86% of the catalytic activity relative to wild-type enzyme |
742512 |
