EC Number   |
Protein Variants |
Reference |
|---|
   1.14.12.22 | F329V |
mutation in oxygenase component. Strong decrease in activity with all substrates tested |
707776 |
| 1.14.12.22 | F329W |
mutation in oxygenase component. Strong decrease in activity with all substrates tested |
707776 |
| 1.14.12.22 | I262A |
mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene |
707776 |
| 1.14.12.22 | I262L |
mutation in oxygenase component. Increase in activity with anthracene |
707776 |
| 1.14.12.22 | I262V |
mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene |
707776 |
| 1.14.12.22 | I262V |
the mutant catalyzes the lateral dioxygenation of carbazole with higher yields than the wild type enzyme |
741682 |
| 1.14.12.22 | I262W |
mutation in oxygenase component. Decrease in activity with carbazole, dibenzo-p-dioxin, anthracene, very low activity with fluorene, fluoranthene |
707776 |
| 1.14.12.22 | more |
expression of the catalytic subunit CarAa is enhanced by induction at a lower cell concentration and temperature and over a longer time, while the expression of subunits CarAc and CarAd is inverse. Best co-expression condition tested is cell concentration at induction of 0.5 (absorbance at 600 nm), 37°C amd 4 h |
725637 |
| 1.14.12.22 | Q282N |
mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene |
707776 |
| 1.14.12.22 | Q282S |
mutation in oxygenase component. Decrease in activity with carbazole, very low activtiy with dibenzo-p-dioxin, anthracene, fluorene, fluoranthene |
707776 |
