EC Number   |
Protein Variants |
Reference |
|---|
   1.13.11.B6 | T579S |
possesses activity profile that is similar to wild-type |
703535 |
| 1.13.11.B6 | V570I |
KM-value for linoleate is 61% of wild-type value, kcat/Km for linoleate is 367% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.6 (compared to 0.8 in reaction with wild-type enzyme) |
396190 |
| 1.13.11.B6 | V570I |
possesses activity profile that is similar to wild-type |
703535 |
| 1.13.11.B6 | V580F |
KM-value for linoleate is 384% of wild-type value, kcat/Km for linoleate is 4% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.9 (compared to 0.8 in reaction with wild-type enzyme) |
396190 |
| 1.13.11.B6 | V582F |
site-directed mutagenesis, mutant substrate specificity compared to the wild-type enzyme, chiral analysis |
742627 |
| 1.13.11.B6 | W523A |
kcat/KM for linoleate hydroperoxidation is reduced by 98% |
703535 |
