EC Number   |
Protein Variants |
Reference |
|---|
    1.13.11.4 | F159Y |
site-directed mutagenesis |
724975 |
| 1.13.11.4 | G106A |
site-directed mutagenesis, in contrast to the wild-type enzyme, mutant G106A oxidizes only gentisate, while 1-hydroxy-2-naphthoate and salicylate are not converted. The mutant shows slightly decreased activity with salicylate, but shows a higher affinity to this substratecompared to the wild-type. Salicylate is coordinated in the G106A variant with the catalytically active Fe(II) ion in an unusual and unproductive manner because of the inability of salicylate to displace a hydrogen bond that is formed between Trp104 and Asp174 in the G106A variant |
-, 724975 |
| 1.13.11.4 | G111A |
site-directed mutagenesis |
-, 724975 |
| 1.13.11.4 | G123N |
75% relative activity compared to the wild type enzyme |
-, 674253 |
| 1.13.11.4 | G164T |
complete loss of activity |
674253 |
| 1.13.11.4 | H108D |
complete loss of activity |
-, 675818 |
| 1.13.11.4 | H108D |
no enzymatic activity |
389991 |
| 1.13.11.4 | H110D |
complete loss of activity |
-, 675818 |
| 1.13.11.4 | H110D |
no enzymatic activity |
389991 |
| 1.13.11.4 | H118D |
complete loss of activity |
675818 |
