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1,4-dihydroxy-2-naphthoate + O2
?
1-hydroxy-2-naphthoate + O2
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
3,4-dichlorosalicylate + O2
?
3,4-dimethylgentisate + O2
?
3,5-dibromosalicylate + O2
?
3,6-dichlorogentisate + O2
?
3-aminosalicylate + O2
(2E,4Z)-2-amino-6-oxohepta-2,4-dienoate
Substrates: -
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
3-bromosalicylate + O2
(2E,4Z)-2-bromo-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
3-bromosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3-chlorogentisate + O2
3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
3-chlorosalicylate + O2
(2E,4Z)-2-chloro-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
3-chlorosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3-ethylgentisate + O2
3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
3-fluorogentisate + O2
3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
3-hydroxybenzoate + O2
3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid
3-hydroxysalicylate + O2
(2E,4Z)-2-hydroxy-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
3-hydroxysalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3-isopropyl-gentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
3-isopropylgentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
3-methylgentisate + O2
3-methyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: Vmax is 10.1% of Vmax for gentisate
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
3-methylsalicylate + O2
(2Z,4Z)-2-methyl-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
3-methylsalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3-O-methylgentisate + O2
?
4-aminosalicylate + O2
(2Z,4E)-3-amino-6-oxohepta-2,4-dienoate
Substrates: -
Products: -
?
4-bromosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
4-chlorogentisate + O2
2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
4-chlorosalicylate + O2
(2Z,4E)-3-chloro-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
4-chlorosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
4-fluorogentisate + O2
2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
4-hydroxysalicylate + O2
(2Z,4E)-3-hydroxy-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
4-hydroxysalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
4-isopropylgentisate + O2
4-hydroxy-2-isopropyl-6-oxo-hepta-2,4-dienedioic acid
4-methylgentisate + O2
4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid
4-methylsalicylate + O2
(2Z,4Z)-3-methyl-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
4-methylsalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
4-O-methylgentisate + O2
?
5-aminosalicylate + O2
(2Z,4E)-4-amino-6-oxohepta-2,4-dienedioate
5-bromosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
5-chlorosalicylate + O2
(2Z,4E)-4-chloro-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
5-chlorosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
5-fluorosalicylate + O2
(2Z,4E)-4-fluoro-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
5-fluorosalicylate + O2
?
5-methylsalicylate + O2
(2Z,4Z)-4-methyl-6-oxohepta-2,4-dienoate
Substrates: low activity
Products: -
?
5-methylsalicylate + O2
?
benzoate + O2
3-hydroxy-6-oxoheptadienoic acid
-
Substrates: -
Products: -
?
cinnamate + O2
?
-
Substrates: -
Products: -
?
gentisate + O2
maleylpyruvate
phenylpropionate + O2
?
-
Substrates: -
Products: -
?
salicylate + O2
2-oxohepta-3,5-dienedioic acid
salicylate + O2
?
-
Substrates: -
Products: -
?
additional information
?
-
1,4-dihydroxy-2-naphthoate + O2
?
-
Substrates: -
Products: -
?
1,4-dihydroxy-2-naphthoate + O2
?
-
Substrates: -
Products: -
?
1,4-dihydroxy-2-naphthoate + O2
?
Substrates: -
Products: -
?
1,4-dihydroxy-2-naphthoate + O2
?
Substrates: -
Products: -
?
1-hydroxy-2-naphthoate + O2
?
-
Substrates: -
Products: -
?
1-hydroxy-2-naphthoate + O2
?
-
Substrates: -
Products: -
?
1-hydroxy-2-naphthoate + O2
?
Substrates: weak activity
Products: -
?
1-hydroxy-2-naphthoate + O2
?
Substrates: weak activity
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: the enzyme is highly specific for 2,5-dihydroxybenzoate
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: the enzyme is highly specific for 2,5-dihydroxybenzoate
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: the enzyme is involbved in the 3-phenylpropionic acid degĀ“radation pathway
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
ir
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
ir
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: i.e. gentisate
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: i.e. gentisate
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: i.e. gentisate
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: i.e. gentisate
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: i.e. gentisic acid
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
ir
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
ir
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
ir
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: narrow substrate specificity
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: narrow substrate specificity
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
-
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-dihydroxybenzoate + O2
maleylpyruvate
Substrates: -
Products: -
?
2,5-xylenol + O2
?
-
Substrates: -
Products: -
?
2,5-xylenol + O2
?
-
Substrates: -
Products: -
?
3,4-dichlorosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3,4-dichlorosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3,4-dimethylgentisate + O2
?
-
Substrates: -
Products: -
?
3,4-dimethylgentisate + O2
?
-
Substrates: -
Products: -
?
3,5-dibromosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3,5-dibromosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3,6-dichlorogentisate + O2
?
-
Substrates: -
Products: -
?
3,6-dichlorogentisate + O2
?
-
Substrates: -
Products: -
?
3-aminosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3-aminosalicylate + O2
?
-
Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: Vmax is 48.5% of Vmax for gentisate
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-bromogentisate + O2
3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-chlorogentisate + O2
3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-chlorogentisate + O2
3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-chlorogentisate + O2
3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-ethylgentisate + O2
3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-ethylgentisate + O2
3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-ethylgentisate + O2
3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-ethylgentisate + O2
3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: Vmax is 39.7% of Vmax for gentisate
Products: -
?
3-ethylgentisate + O2
3-ethyl-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: Vmax is 39.7% of Vmax for gentisate
Products: -
?
3-fluorogentisate + O2
3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-fluorogentisate + O2
3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-fluorogentisate + O2
3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-fluorogentisate + O2
3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-fluorogentisate + O2
3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: Vmax of recombinant enzyme is 20.5% higher compared to gentisate
Products: -
?
3-fluorogentisate + O2
3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-fluorogentisate + O2
3-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: Vmax of recombinant enzyme is 20.5% higher compared to gentisate
Products: -
?
3-hydroxybenzoate + O2
3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: minor pathway
Products: -
?
3-hydroxybenzoate + O2
3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-hydroxybenzoate + O2
3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-hydroxybenzoate + O2
3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: main pathway
Products: -
?
3-hydroxybenzoate + O2
3,4-dihydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-hydroxybenzoate + O2
?
-
Substrates: -
Products: -
?
3-hydroxybenzoate + O2
?
-
Substrates: -
Products: -
?
3-isopropyl-gentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-isopropyl-gentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-isopropyl-gentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-isopropyl-gentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-isopropylgentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-isopropylgentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-isopropylgentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-isopropylgentisate + O2
4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: Vmax is 57.1% of Vmax for gentisate
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-methylgentisate + O2
4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
3-O-methylgentisate + O2
?
-
Substrates: -
Products: -
?
3-O-methylgentisate + O2
?
-
Substrates: -
Products: -
?
4-chlorogentisate + O2
2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
4-chlorogentisate + O2
2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
?
4-chlorogentisate + O2
2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
-
Substrates: -
Products: -
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4-chlorogentisate + O2
2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
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4-chlorogentisate + O2
2-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
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4-fluorogentisate + O2
2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
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4-fluorogentisate + O2
2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
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4-fluorogentisate + O2
2-fluoro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid
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4-hydroxybenzoate + O2
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4-hydroxybenzoate + O2
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4-isopropylgentisate + O2
4-hydroxy-2-isopropyl-6-oxo-hepta-2,4-dienedioic acid
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4-isopropylgentisate + O2
4-hydroxy-2-isopropyl-6-oxo-hepta-2,4-dienedioic acid
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4-methylgentisate + O2
4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid
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4-methylgentisate + O2
4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid
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4-methylgentisate + O2
4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid
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4-methylgentisate + O2
4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid
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4-methylgentisate + O2
4-hydroxy-2-methyl-6-oxo-hepta-2,4-dienedioic acid
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4-O-methylgentisate + O2
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4-O-methylgentisate + O2
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5-aminosalicylate + O2
(2Z,4E)-4-amino-6-oxohepta-2,4-dienedioate
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5-aminosalicylate + O2
(2Z,4E)-4-amino-6-oxohepta-2,4-dienedioate
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5-aminosalicylate + O2
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5-aminosalicylate + O2
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5-aminosalicylate + O2
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5-aminosalicylate + O2
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Substrates: a gentisate 1,2-dioxygenase with an extremely relaxed substrate specificity is responsible for the unusual oxidation of salicylate by Pseudoaminobacter salicylatoxidans BN12. Relative activities with salicylate, 5-aminosalicylate, 1-hydroxy-2-naphthoate, and gentisate with both preparations are about 1:10:40:120
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5-aminosalicylate + O2
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Substrates: kcat/KM is less than 1% of the kcat/Km for gentisate
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5-aminosalicylate + O2
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5-aminosalicylate + O2
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Substrates: a gentisate 1,2-dioxygenase with an extremely relaxed substrate specificity is responsible for the unusual oxidation of salicylate by Pseudoaminobacter salicylatoxidans BN12. Relative activities with salicylate, 5-aminosalicylate, 1-hydroxy-2-naphthoate