EC Number   |
|---|
   7.5.2.1 | crystal structure analysis |
| 7.5.2.1 | crystal structure analysis of full transporter MalFGK2 and of the MalF subunit |
| 7.5.2.1 | crystal structure analysis, e.g. of the crystal structure of the complex in the presence of ATP and MalE, containing MalK mutant E159Q, is resolved at 2.8 A, revealing that MalFG are in a periplasmically open state |
| 7.5.2.1 | enzyme IIA in complex with the maltose transporter, vapor diffusion method, using 100 mM sodium cacodylate pH 5.6, 13% (w/v) PEG monomethylether 2000 and 100 mM NDSB-256 |
| 7.5.2.1 | MalE domain, sitting drop vapor diffusion method, using 100 mM BisTris, pH 5.5, 0.2 M LiSO4, 25% (w/v) PEG 3350 |
| 7.5.2.1 | MalK crystal structure analysis in the semi-open, nucleotide-free, the open nucleotide-free, the ATP-bound, and the ADP-bound states |
| 7.5.2.1 | pre-translocation, outward- and inward-facing crystal structure analysis of enzyme complex with or without bound maltose binding protein and bound maltose and ATP, overview |
| 7.5.2.1 | purified native MalFGK2 and and selenium methionine-labeled MalFGK2, in 20 mM Tris, pH 8.0, 50 mM NaCl, and 0.03% n-undecyl-beta-D-maltopyranoside, addtion of 5 mM 2-mercaptoethanol and 5 mM DTT for the SeMet-enzyme. Mixing of 1 mg/ml protein and reservoir solution, containing 11.5% PEG 4000, 0.1M N-(2-acetamido)iminodiacetic acid, pH 6.5, 0.1 M NaCl, and 0.1 M Li2SO4, in a 2:1 ratio, at 20°C in sitting drops, crystals appear after 24 h and grow to full size within 1 week, X-ray diffraction structure determination and anaylsis at 2.8 A resolution |
