Information on EC 7.5.2.1 - ABC-type maltose transporter

P02916; P68183; P68187; P0AEX9

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MalG511

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the binding-protein-independent mutant L135F/I154S of Escherichia coli maltose transporter MalFGK2 retains specificity for maltose and shows a high basal ATPase activity in the absence of maltose binding protein

MalK

MalK2

malK3

Q9X0S9; Q9X2F5

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MalP

-

MalT

-

MALT2

MALT4

maltose ABC importer

P02916; P68183; P68187; P0AEX9

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767960

maltose ABC transporter

maltose ABC transporter MALFGK2-E

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698908

maltose ABC-type transporter

Thermotoga maritima

Q9X0S9; Q9X2F5

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746843

maltose ATP binding cassette transporter

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696314

maltose ATP-binding cassette importer

maltose ATP-binding cassette transporter

maltose importer

maltose transporter

maltose transporter ATPase

maltose transporter MalFGK2

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maltose-specific enzyme IICB

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maltose-transporting ATPase

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maltose/maltodextrin ABC transporter

maltose/maltodextrin transport system

P02916; P68183; P68187; P0AEX9

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767960

Malx1

MuSK

proton-maltose symporter

additional information

ATP + H2O + maltose-[maltose-binding protein][side 1] = ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]

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hydrolysis of phosphoric ester

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transmembrane transport

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ATP phosphohydrolase (ABC-type, maltose-importing)

An ATP-binding cassette (ABC) type transporter, characterized by the presence of two similar ATP-binding domains/proteins and two integral membrane domains/proteins. The enzyme, found in bacteria, interacts with an extracytoplasmic substrate binding protein and mediates the import of maltose and maltose oligosaccharides.

9000-83-3

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ATP + H2O

ADP + phosphate

ATP + H2O + maltose-[maltose-binding protein][side 1]

ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]

19 entries

ATP + H2O + maltose-[maltose-binding protein][side 1] + H+[side1]

ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1] + H+[side2]

ATP + H2O + maltose/out

ADP + phosphate + maltose/in

31 entries

additional information

?

-

15 entries

ATP + H2O + maltose-[maltose-binding protein][side 1]

ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1]

19 entries

ATP + H2O + maltose-[maltose-binding protein][side 1] + H+[side1]

ADP + phosphate + maltose[side 2] + [maltose-binding protein][side 1] + H+[side2]

ATP + H2O + maltose/out

ADP + phosphate + maltose/in

16 entries

additional information

?

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ATP

additional information

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maltose binding protein

656223

-

Mg2+

acarbose

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Aes

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antagonizing maltotriose binding by MalT

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alpha-cyclodextrin

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Salmonella enterica subsp. enterica serovar Typhimurium

antibody 4H12

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recognizes the peptide 111-NQRVNQVAEVLQL-123

655437

-

antibody 5B5

Salmonella enterica subsp. enterica serovar Typhimurium

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recognizes the peptide 53-ETITSGDLTRM-67

655437

-

beta-cyclodextrin

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carbonyl cyanide m-chlorophenyl hydrazone

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completely inhibits maltose release

Salmonella enterica subsp. enterica serovar Typhimurium

EIIAGlc

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when glucose as preferred carbon source is transported, EIIAGlc becomes predominantly unphosphorylated and allosterically inhibits several permeases, including MalFGK2, which possesses a specific binding site in region III of EIIAGlc, in a process termed inducer exclusion involving the N-terminal part of the inhibitor, binding surface, overview

687505

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enzyme IIA Glc

Salmonella enterica subsp. enterica serovar Typhimurium

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654822

-

enzyme IIA(Glc)

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inhibits the maltose ATP-binding cassette transporter MalE-FGK2 by interaction with the nucleotide-binding and -hydrolyzing subunit MalK

747108

-

enzyme IIAglc

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656157

-

gamma-cyclodextrin

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MalK

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0.008 mM, in the absence of maltotriose 80% inhibition, no inhibition in the presence of 0.1 mM maltotriose

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maltose binding protein

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inhibitory above 0.05 mM

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maltotetraose

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MalY

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antagonizing maltotriose binding by MalT

-

monensin

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strongly inhibits maltose release

N,N'-dicyclohexylcarbodiimide

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strongly inhibits maltose release

NaN3

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FGK2 complex

nigericin

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completely inhibits maltose release

p-chloromercuriphenyl sulfonic acid

-

FGK2 complex

vanadate

additional information

-

not inhibited by oligomycin and antimycin A

-

MalE

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MalF

-

MalF stimulates the substrate transport activity through its second periplasmic loop, overview

720017

-

maltose

maltose binding protein

-

low levels of maltose binding protein stimulate enzyme activity

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maltose-binding protein

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maltotetraose

5fold stimulation of ATPase activity at 0.6 mM

maltotriose

4fold stimulation of ATPase activity at 0.6 mM

substrate-binding protein MalE

-

binds with high-affinity to MalFGK2 and facilitate the acquisition of maltose

720859

-

verapamil

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stimulates detergent-soluble maltose transporter, increases ATPase activity and lowers affinity for ATP

additional information

5 entries

-

0.1 - 0.33

ATP

0.0005 - 0.0075

maltose

1.2 - 40.9

maltose-[maltose-binding protein][side 1]

-

0.3 - 7.8

ATP

0.011

-

246873

0.13

Q70HW1

-

654879

0.22

-

Salmonella enterica subsp. enterica serovar Typhimurium

1.25

-

654822

additional information

Streptomyces avermitilis

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avermectin production by wild-type and malEFG-a utant strains, overview

5

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the acidic condition (pH 5.0) is essential for maltose transport

6.2

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maltose transport assay

7.4

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assay at

720017

7.5

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assay at

8

22

assay at, enzyme reconstituted in proteoliposomes

23

-

assay at

25

-

assay at

720017

37

70

-

assay at

-

-

-

Bdellovibrio bacteriovorus HD100

UniProt

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UniProt

-

-

Chlorella variabilis F36-ZK

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Corynebacterium glutamicum

-

-

734077

Q8NMV1

SwissProt

Corynebacterium glutamicum ATCC 13032

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SwissProt

genes malEFG

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Lactococcus lactis IL1403

genes malEFG

718597

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no activity in Chlorella vulgaris strain NIES-227

-

-

Saccharomyces cerevisiae CEN.PK2-1D

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749334, 766711

-

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Saccharomyces cerevisiae FY834

-

-

-

-

-

Saccharomyces eubayanus

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747855, 766711

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Saccharomyces eubayanus CBS 12357T

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747855

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Saccharomyces eubayanus VTT-C12902

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Saccharomyces pastorianus

-

-

-

Saccharomyces pastorianus A-63015

-

-

Saccharomyces pastorianus A-75060

-

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serovar typhimurium

Salmonella enterica subsp. enterica serovar Typhimurium

-

-

246881, 654822, 655437, 687505, 696772, 713059

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Streptomyces avermitilis

contains the maltose ABC transporter system encoded by malEFG-a

Streptomyces avermitilis ATCC 31267

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contains the maltose ABC transporter system encoded by malEFG-a

-

-

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Thermotoga maritima

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746843

Q9X0S9; Q9X2F5

UniProt

cell wall

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the majority of the maltose binding protein is distributed throughout the cell wall, while only smaller quantities are located in the cytoplasmic membrane

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P02916; P68183; P68187; P0AEX9

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Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

evolution

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the enzyme is a member of the ATP-binding cassette superfamily

malfunction

metabolism

physiological function

additional information

Escherichia coli K-12

170000

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tetrameric FGK2 complex, gel filtration

246873

42000

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x * 42000, SDS-PAGE

42977

Q70HW1

x * 42977, calculated from nucleotide sequence

?

dimer

multimer

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the maltose transporter consists of a periplasmic maltose binding protein and a multisubunit membrane transporter, MalFGK2

additional information

11 entries

glycoprotein

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MalE domain, sitting drop vapor diffusion method, using 100 mM BisTris, pH 5.5, 0.2 M LiSO4, 25% (w/v) PEG 3350

crystal structure analysis of full transporter MalFGK2 and of the MalF subunit

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696314

crystal structure analysis, e.g. of the crystal structure of the complex in the presence of ATP and MalE, containing MalK mutant E159Q, is resolved at 2.8 A, revealing that MalFG are in a periplasmically open state

713059

enzyme IIA in complex with the maltose transporter, vapor diffusion method, using 100 mM sodium cacodylate pH 5.6, 13% (w/v) PEG monomethylether 2000 and 100 mM NDSB-256

734773

MalK crystal structure analysis in the semi-open, nucleotide-free, the open nucleotide-free, the ATP-bound, and the ADP-bound states

685001

pre-translocation, outward- and inward-facing crystal structure analysis of enzyme complex with or without bound maltose binding protein and bound maltose and ATP, overview

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721055

purified native MalFGK2 and and selenium methionine-labeled MalFGK2, in 20 mM Tris, pH 8.0, 50 mM NaCl, and 0.03% n-undecyl-beta-D-maltopyranoside, addtion of 5 mM 2-mercaptoethanol and 5 mM DTT for the SeMet-enzyme. Mixing of 1 mg/ml protein and reservoir solution, containing 11.5% PEG 4000, 0.1M N-(2-acetamido)iminodiacetic acid, pH 6.5, 0.1 M NaCl, and 0.1 M Li2SO4, in a 2:1 ratio, at 20°C in sitting drops, crystals appear after 24 h and grow to full size within 1 week, X-ray diffraction structure determination and anaylsis at 2.8 A resolution

Salmonella enterica subsp. enterica serovar Typhimurium

crystal structure analysis

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713059

A96W/I329W

C40S/S83C

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site-directed mutagenesis, the mutant shows altered subunit interactions in the maltose ABC transporter MALFGK2-E

698908

D380G

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substitution of aspartate for glycine in the maltose-binding site of MalF likely generates a futile cycle by preventing maltose from binding to MalFGK2 during the catalytic cycle

DELTAE229

P02916; P68183; P68187; P0AEX9

deletion of E229 leads to the failure in complex assembly and maltose transport

E159

the E159Q substitution in MalK prevents ATP from being hydrolysed. This results in an ATP-bound structure, which is thought to represent the transition state for ATP hydrolysis. The crystal shows an open (unliganded) MalE bound to the periplasmic side of the complex, a maltose molecule bound solely to a transmembrane region of MalF, and a closed conformation of the ATP-bound MalK(E59Q)2 subunits

713059

E159Q

E313C

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leads to a disulfide bond, no effect to ATPase activity

656157

L135F/I154S

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the binding-protein-independent mutant of Escherichia coli maltose transporter MalFGK2 retains specificity for maltose and shows a high basal ATPase activity in the absence of maltose binding protein

S205C/S252C

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site-directed mutagenesis, the mutant shows altered subunit interactions in the maltose ABC transporter MALFGK2-E

698908

T31C/S205C

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site-directed mutagensis, formation of a crosslink betwwen the mutant subunits

T31C/T177C

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site-directed mutagenesis, the distance between MalF mutant and MalE T31C mutant changes from 10 A to 5 A upon binding of ATP or maltose, with a less pronounced result, and is reset to 10 A after hydrolysis of one ATP

T80C/S205C

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site-directed mutagenesis, the mutation of subunit MalF mutant S205C does not affect the interaction with MalE mutant T80C

W230C

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site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein

W340C

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site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein

W62C

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site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein, but retains good function in maltose transport and MBP-stimulated ATPase activities

Y155C

-

site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein

Y325S

P02916; P68183; P68187; P0AEX9

the mutant retains 52% of the transport activity of the wild type enzyme

Y341C

-

site-directed mutagenesis, mutation in the maltose binding protein, the mutant shows altered kinetics of substrate binding compared to the wild-type maltose binding protein, but retaines good function in maltose transport and MBP-stimulated ATPase activities

Y383S

P02916; P68183; P68187; P0AEX9

the mutation of subunit MalF leads to a change of the substrate specificity from maltose to maltoheptaose

D123A

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the mutant shows wild type activity

E120A

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the mutant shows strongly reduced activity compared to the wild type enzyme

E120A/D123A/E167A

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the mutations eliminate uphill maltose transport

E120N/D123N/E167N

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the mutations eliminate uphill maltose transport

E120Q

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the mutant shows reduced activity compared to the wild type enzyme

E120Q/D123Q/E167Q

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the mutations eliminate uphill maltose transport

E167A

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the mutant shows strongly reduced activity compared to the wild type enzyme

E167Q

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the mutant shows severely reduced activity compared to the wild type enzyme

D123A

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the mutant shows wild type activity

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E120A

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the mutant shows strongly reduced activity compared to the wild type enzyme

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E120Q

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the mutant shows reduced activity compared to the wild type enzyme

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E167A

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the mutant shows strongly reduced activity compared to the wild type enzyme

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E167Q

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the mutant shows severely reduced activity compared to the wild type enzyme

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T31C/S205C

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site-directed mutagensis, formation of a crosslink betwwen the mutant subunits

T31C/T177C

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site-directed mutagenesis, the distance between MalF mutant and MalE T31C mutant changes from 10 A to 5 A upon binding of ATP or maltose, with a less pronounced result, and is reset to 10 A after hydrolysis of one ATP

T80C/S205C

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site-directed mutagenesis, the mutation of subunit MalF mutant S205C does not affect the interaction with MalE mutant T80C

Salmonella enterica subsp. enterica serovar Typhimurium

G137A

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mutation in MalK, fails to restore a functional transport complex in vivo, mutation increases the repressing activity of MalK on other maltose-regulated genes, loss of ability to hydrolyze ATP, but still displays nucleotide-binding activity

Salmonella enterica subsp. enterica serovar Typhimurium

G137T

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mutation in MalK, fails to restore a functional transport complex in vivo, mutation increases the repressing activity of MalK on other maltose-regulated genes, loss of ability to hydrolyze ATP, but still displays nucleotide-binding activity

Salmonella enterica subsp. enterica serovar Typhimurium

G137V

-

mutation in MalK, fails to restore a functional transport complex in vivo, mutation increases the repressing activity of MalK on other maltose-regulated genes, loss of ability to hydrolyze ATP, but still displays nucleotide-binding activity

Q140K

Salmonella enterica subsp. enterica serovar Typhimurium

Q140L

-

mutation in MalK, fails to restore a functional transport complex in vivo, mutation increases the repressing activity of MalK on other maltose-regulated genes, fails to hydrolyze ATP and exhibits a strong intrinsic resistance to trypsin in the absence of MgATP, suggesting a drastically altered conformation

Salmonella enterica subsp. enterica serovar Typhimurium

Q140N

-

mutation in MalK, fails to restore a functional transport complex in vivo, mutation increases the repressing activity of MalK on other maltose-regulated genes, ATPase activity and MgATP-induced changes in tryptic cleavage pattern similar to those of wild-type. Mutant transport complexes containing MalKQ140N variant, when studied in proteoliposomes, are severely impaired in MalE-maltose-stimulated ATPase activity

additional information

maltose-binding protein effectively stabilizes the partially closed conformation of the enzyme

Salmonella enterica subsp. enterica serovar Typhimurium

-

748866

MgATP2-, binding protects against trypsin inactivation in wild-type enzyme in in G137 mutants

-

-

cobalt resin column chromatography and Talon resin column chromatography

734727

cobalt-affinity column chromatography and Superdex 200 gel filtration

734773

maltose binding protein MalE

-

Ni-NTA-agarose microcolumn chromatography

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Ni2+-chelating affinity gel chromatography and gel filtration

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Ni2+-chelating column chromatography and Superdex 200HR gel filtration

Salmonella enterica subsp. enterica serovar Typhimurium

-

733387

purification of recombinant subunits

-

654822

recombinant His-tagged enzyme from Escherichia coli strain JM109

recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by nickel affinity chromatography and ultrafiltration

recombinant wild-type and mutant MalFGK2 from Escherichia coli strain AD110 by cobalt affinity chromatography and gel filtration

Talon-HiTrap resin column chromatography, and Superdex 200 gel filtration

wild-type strain and mutant strain that does not require maltose-binding protein for transport

-

246876

with the aid of a glutathione S-transferase molecular tag

-

-

Corynebacterium glutamicum

expressed in a maltose-transport-deficient Saccharomyces cerevisiae strain IMZ616

Saccharomyces eubayanus

-

747855

expressed in Corynebacterium glutamicum strain IMcg2701

Q8NMV1

734077

expressed in Escherichia coli AD126 cells

-

expressed in Escherichia coli BL21 Star (DE3) cells

734773

expressed in Escherichia coli BL21 T1 DE3 (pLysS) cells

expressed in Escherichia coli BL21(DE3) cells

-

733387

expressed in Escherichia coli KM9 cells

P68187; P68183; P02916

748209

expressed in Escherichia coli strain RB791/pMBPHis6x

-

expression in Escherichia coli

expression in Escherichia coli strain AD110

expression in strain HN741

-

656157

genes malEFG-a, encoding the maltose ABC transporter system, transcriptional analysis of malE-a by RT-PCR, and malEFG-a gene replacement cloning

Streptomyces avermitilis

-

maltose-binding protein MalE expressed in Escherichia coli

-

overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain JM109

overexpression of MalF in Escherichia coli strain BL21(DE3)

-

696314

recombinant His-tagged enzyme expression in Escherichia coli strain JM109

the type I maltose ABC importer is encoded within the maltose regulon

enzyme genes are induced by maltose

enzyme genes are repressed by glucose

expression of malE-a is induced by maltose

expression of malE-a is strongly repressed by glucose