EC Number   |
Reference |
|---|
    6.1.1.22 | - |
385 |
| 6.1.1.22 | AsnRS complexed with asparaginyl-adenylate Asn-AMP, or free AsnRS and AsnRS complexed with an Asn-AMP analog Asn-SA, sitting-drop vapor diffusion method at 20°C, 0.001 ml protein solution is mixed with an equal volume of reservoir solution containing 25 mM sodium cacodylate buffer, pH 6.5, 50 mM sodium acetate trihydrate, and 7.5% w/v PEG 8000, equilibration against 0.5 ml reservoir solution, for ligand complex formation the crystals are soaked in a reservoir solution, containing 1.5 mM AMP-PNP, 1.5 mM asparagine, and 2 mM MgCl2, for approximately 10 h, followed by a reservoir solution containing 25% 2-methyl-2,4-pentanediol for a few seconds, X-ray diffraction strucure determination and analysis at 1.45 A, and 1.98 A and 1.8 A resolution, respectively |
675391 |
| 6.1.1.22 | AsnRS with bound asparaginyladenylate, AsnAMP, X-ray diffraction structure anaylsis at 2.6 A resolution |
704835 |
| 6.1.1.22 | identification of peptidyl regions that are surface-accessible and available for antibody binding |
695019 |
| 6.1.1.22 | molecular modeling of structure |
746169 |
| 6.1.1.22 | purified apo-AsnRS, AsnRS in complex with AsnAMS, and in complex with Mg2+, ATP, and l-Asp-beta-NOH, X-ray diffraction structure determination and analysis at 1.9-2.4 A resolution |
715924 |
| 6.1.1.22 | purified dimeric enzyme with bound Mg2+ and a non-hydrolyzable analogue of asparaginyl adenylate, ASNAMS, X-ray diffraction structure determination and analysis at 1.9 A resolution, modeling |
675081 |
| 6.1.1.22 | recombinant enzyme, native or complexed withATP and asparaginyl-adenylate, 3 different crystal forms, X-ray diffraction structure determination at 2.6 A resolution and analysis |
650960 |
| 6.1.1.22 | sitting drop vapor diffusion method, using 100 mM Tris-HCl (pH 7.0), 0.2 M NaCl, and 32% (w/v) polyethylene glycol 3350 |
728147 |
