Application | Comment | Organism |
---|---|---|
drug development | Brugia malayi asparaginyl-tRNA synthetase, a target for anti-parasitic drug design | Brugia malayi |
Crystallization (Comment) | Organism |
---|---|
purified dimeric enzyme with bound Mg2+ and a non-hydrolyzable analogue of asparaginyl adenylate, ASNAMS, X-ray diffraction structure determination and analysis at 1.9 A resolution, modeling | Brugia malayi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
asparaginyl sulfamoyl adenylate | i.e. ASNAMS, a non-hydrolyzable analogue of asparaginyl adenylate | Brugia malayi | |
asparaginyl sulfamoyl adenylate | i.e. ASNAMS, a non-hydrolyzable analogue of asparaginyl adenylate | Homo sapiens | |
cycloadenosine | - |
Brugia malayi | |
L-aspartate-beta-hydroxamate adenylate | i.e. LBHAMP | Brugia malayi | |
LCM01 | long side-chain derivative of variolin B | Brugia malayi | |
LCM01 | long side-chain derivative of variolin B | Homo sapiens | |
LCM02 | long side-chain derivative of variolin B | Brugia malayi | |
LCM02 | long side-chain derivative of variolin B | Homo sapiens | |
additional information | no inhibition by NSC35467 at 0.2 mM | Homo sapiens | |
NSC114691 | i.e. 8-chloro-3-(hydroxy(oxido)amino)-6-phenanthridinol | Brugia malayi | |
NSC114691 | i.e. 8-chloro-3-(hydroxy(oxido)amino)-6-phenanthridinol | Homo sapiens | |
NSC12156 | i.e. N1,N3-bis(4-amino-2-methyl-6-quinolinyl)-2,2-dimethylmalonamide, 47% inhibition at 0.2 mM, docking mode and binding structure, overview | Brugia malayi | |
NSC12156 | i.e. N1,N3-bis(4-amino-2-methyl-6-quinolinyl)-2,2-dimethylmalonamide, 54% inhibition at 0.2 mM, docking mode and binding structure, overview | Homo sapiens | |
NSC35467 | i.e. 2-(3-methyl-1 lambda5-pyridin-1-yl)-1-(2-phenanthryl)ethanone, 53% inhibition at 0.2 mM | Brugia malayi | |
NSC363624 | i.e. 4-(3-(4-amino-6-isopropenyl-1,3,5-triazin-2-yl)phenyl)-6-isopropenyl-1,3,5-triazin-2-ylamine, 50% inhibition at 0.025 mM | Brugia malayi | |
NSC363624 | i.e. 4-(3-(4-amino-6-isopropenyl-1,3,5-triazin-2-yl)phenyl)-6-isopropenyl-1,3,5-triazin-2-ylamine, 80% inhibition at 0.025 mM | Homo sapiens | |
rishirilide B | CSD code CUQZUJ, isolated from Streptomyces rishiriensis, has antithrombotic activity through selective alpha2-macroglobulin inhibition, leading to the activation of plasmin | Brugia malayi | |
rishirilide B | - |
Homo sapiens | |
S-methyl-deoxyvariolin B | i.e. SMEVAR | Brugia malayi | |
variolin B | - |
Brugia malayi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | binding structure | Brugia malayi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brugia malayi | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-asparagine + tRNAAsn | with the aminoacylation step E + AA + ATP = E(AA-AMP) + diphosphate, and the amidation step E(AA-AMP) + tRNA = E+AA-tRNA + AMP | Brugia malayi | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
r | |
ATP + L-asparagine + tRNAAsn | with the aminoacylation step E + AA + ATP = E(AA-AMP) + diphosphate, and the amidation step E(AA-AMP) + tRNA = E+AA-tRNA + AMP | Homo sapiens | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
r | |
additional information | active site and ligand binding structure and mechanism, overview | Brugia malayi | ? | - |
? | |
additional information | active site and ligand binding structure and mechanism, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | structure analysis using crystal structure | Homo sapiens |
dimer | structure analysis using crystal structure, modeling the conformational flexibility of Brugia AsnRS, overview | Brugia malayi |
Synonyms | Comment | Organism |
---|---|---|
AsnRS | - |
Brugia malayi |
AsnRS | - |
Homo sapiens |
Asparaginyl-tRNA synthetase | - |
Brugia malayi |
Asparaginyl-tRNA synthetase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Brugia malayi | |
ATP | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0017 | - |
- |
Homo sapiens | asparaginyl sulfamoyl adenylate | |
0.004 | - |
- |
Brugia malayi | L-aspartate-beta-hydroxamate adenylate | |
0.0045 | - |
- |
Brugia malayi | asparaginyl sulfamoyl adenylate | |
0.025 | - |
- |
Brugia malayi | NSC363624 | |
0.05 | - |
- |
Homo sapiens | NSC114691 | |
0.065 | - |
- |
Brugia malayi | NSC114691 | |
0.123 | - |
- |
Brugia malayi | LCM02 | |
0.173 | - |
- |
Brugia malayi | LCM01 | |
0.24 | - |
- |
Brugia malayi | rishirilide B | |
0.4 | - |
- |
Homo sapiens | human angiotensinogen | |
0.5 | - |
above | Homo sapiens | LCM01 | |
1 | - |
above | Homo sapiens | LCM02 |