EC Number   |
|---|
   4.6.1.23 | - |
| 4.6.1.23 | 15N-1H residual dipolar coupling NMR, alkyl poly(ethylene glycol)/alcohol mixture, 1 mM, pH 6.0, 25°C, 24 Hz |
| 4.6.1.23 | alpha-sarcin three-dimensional structure determined by X-ray diffraction of single crystals of the toxin |
| 4.6.1.23 | circular dichroism studies. The protein contains about 40% of periodic structures, mainly located at both extremes of the polypeptide chain. beta-Turns and aperiodic conformation appear at the central part of the molecule |
| 4.6.1.23 | homology modeling of structure. Ageritin has a structural core consisting of an antiparallel beta-sheet and an adjacent long alpha-helix, typical of ribotoxins and RNase T1 family |
| 4.6.1.23 | modeling of structure |
| 4.6.1.23 | modeling of the catalytic metal binding site of ageritin, made of the putative triad Asp68, Asp70 and His77. Ageritin has the structure and function of an RNase but a Mg2+/Zn2+-dependent mechanism of action |
| 4.6.1.23 | to 2.1 A resolution. The tertiary structure of the substrate RNA is important in protein-RNA recognition, fitting closely into the concavity of the presumed binding site |
