EC Number |
---|
4.1.2.14 | - |
4.1.2.14 | apoform and in complex with 3-phosphoglycerate, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 8,000 and 0.1 M Bis-Tris (pH 5.5) for the apoform or 1.6 M ammonium sulfate, 0 .1 M CAPS (pH 10.5), and 0.2 M lithium sulfate for the 3-phosphoglycerate-bound form |
4.1.2.14 | enzyme in complex with pyruvate, 2-dehydro-3-deoxy-D-gluconate or 2-dehydro-3-deoxy-D-galactonate, determination of the structural basis for substrate promiscuity |
4.1.2.14 | hanging drop vapor diffusion method |
4.1.2.14 | hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 3350, and 0.1 M bis-tris pH 5.5 |
4.1.2.14 | in complex with 2-dehydro-3-deoxy-6-phospho-D-gluconate |
4.1.2.14 | microdialysis against sequentially higher concentrations of ammonium sulfate |
4.1.2.14 | sitting drop vapor diffusion method |
4.1.2.14 | structure at 2.8 A resolution |
4.1.2.14 | the crystal structure of the apoprotein is determined to a resolution of 2.0 A, the structure of the protein covalently linked to pyruvate is determined to 2.2 A |