EC Number   |
Reference  |
|---|
   3.4.11.9 | 8 mg/ml purified recombinant enzyme in Tris, pH 8.5, hanging drop vapour diffusion method, 0.003 ml mixed with 0.002 ml reservoir solution containing 25% PEG 4000, 0.1 M Tris-HCl, pH 8.0, 0.2 M sodium acetate, and 1 mM MnCl2, 1 month at 4°C, cryoprotectant is 2-methyl-2,4-pentanediol, X-ray diffraction structure determination and analysis at 2.4 A resolution, modeling |
649232 |
| 3.4.11.9 | in complex with inhibitor apstatin |
667056 |
| 3.4.11.9 | Mn(II)-form of enzyme and substituted with Mg2+, Zn2+, Ca2+, Na+ and apo-enzyme in complex with L-Val-L-Pro-L-Leu |
667633 |
| 3.4.11.9 | mutants H243A, D260A, D271A, H350A, H354A, H361A, E383A |
667743 |
| 3.4.11.9 | mutants E383A, H361A and H243A in complex with substrate L-Val-L-Pro-L-Leu. Substrate interacts with one of the active site metal ions via its terminal amino group |
683096 |
| 3.4.11.9 | hanging drop vapour diffusion method, using 20% (v/v) polyethylene glycol 400, 0.15 M CaCl2, and 100 mM HEPES (pH 7.5) |
698788 |
| 3.4.11.9 | XPD43 is crystallized to 1.83 A resolution using the microbatch-under-oil technique |
731082 |
| 3.4.11.9 | purified recombinant enzyme PfAPP both unliganded and in complex with inhibitor apstatin, hanging drop vapor diffusion method, mixing of 2:1 vl/v ratio of 6 mg/ml protein solution with reservoir containing 40-50% 2-methyl-2,4-pentanediol, and 0.1 M sodium cacodylate, pH 5.6-6.2, 3-7 days, X-ray diffraction structure determination and analysis at 2.30-2.35 A resolution. Crystals of the apstatin:PfAPP complex are obtained by soaking unliganded crystals in mother liquor supplemented with 2 mM apstatin for 1 h. For the unliganded PfAPP structure, the molecular replacement search model is prepared from the structure of human APP1 (PDB ID 3CTZ) |
752716 |
| 3.4.11.9 | oil microbatch method |
753576 |
| 3.4.11.9 | purified recombinant His-tagged enzyme by sitting drop vapour diffusion method, crystallization buffer containing 17.5% PEG 3350, 0.1 M bis-tris propane, pH 8.5, and 0.2 M sodium malonate is mixed with an equal volume of 3 mg/ml protein in 10 mM Tris/HCl, pH 8.0, and 150 mM NaCl,16°C, X-ray diffraction structure determination and analysis at 1.93 A resolution |
753579 |
