EC Number   |
|---|
    3.1.3.15 | 25% (w/v) polyethylene glycol 3350, 0.2M MgCl2 and 0.1 M Tris-HCl (pH 8.5) by the sitting drop vapor diffusion, or 30% (w/v) polyethylene glycol monomethyl ether, 0.05 M CaCl2, and 0.1 M Bis-Tris (pH 6.5) by the micro-batch method |
| 3.1.3.15 | microbatch crystallization method |
| 3.1.3.15 | purified enzyme in complex with products histidiol or phosphate, or substrate histidinol phosphate with Mg2+, or Lys-CH2-Cys, sitting drop vapor diffusion, mixing of 19 mg/ml protein solution with crystallization solution containing 15% PEG 3350, and 0.2 M diammonium hydrogen phosphate, pH 8.0, or 30% PEG 3350, 0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium acetate, and 10 mM magnesium acetate, over the reservoir supplemented with 100 mM formaldehyde to complete cross-linking between Lys158 and Cys245 and vitrified in Paraton-N, X-ray diffraction structure determination and analysis at 1.19-1.36 A resolution |
| 3.1.3.15 | purified recombinant wild-type enzyme free or in complex with substrate histidinol phosphate, hanging drop vapor diffusion method, mixing of 0.0015 ml of protein solution containing 0.1 mM Zn2+ and 7.5 mM substrate for the complexed crystals, with 0.015 ml of reservoir solution containing 1 M ammonium sulfate, and 750 nl additive which is praseodymium (III) acetate hydrate, 35-40 days, 23°C, X-ray diffraction structure determination and analysis at 1.95 A and 2.90 A, respectively |
| 3.1.3.15 | sitting drop vapor diffusion method, using 25% (w/v) PEG 4000, 0.1 M sodium acetate (pH 4.6), and 0.2 M ammonium sulfate |
| 3.1.3.15 | two crystal forms by the handing-drop vapour-diffusion technique |
| 3.1.3.15 | vapour diffusion method with 0.68 M sodium malonate and 100 mM Tris-HCl (pH 8.5) |
